ID   MLPF_BORBU              Reviewed;         149 AA.
AC   Q9S0B7;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Lipoprotein MlpF {ECO:0000303|PubMed:10948116};
DE   Flags: Precursor;
GN   Name=mlpD {ECO:0000303|PubMed:10948116}; OrderedLocusNames=BB_M28;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OG   Plasmid cp32-6.
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=10672174; DOI=10.1046/j.1365-2958.2000.01698.x;
RA   Casjens S., Palmer N., van Vugt R., Huang W.M., Stevenson B., Rosa P.,
RA   Lathigra R., Sutton G.G., Peterson J.D., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Gwinn M.L., White O., Fraser C.M.;
RT   "A bacterial genome in flux: the twelve linear and nine circular
RT   extrachromosomal DNAs in an infectious isolate of the Lyme disease
RT   spirochete Borrelia burgdorferi.";
RL   Mol. Microbiol. 35:490-516(2000).
RN   [3]
RP   FUNCTION, ANTIGENICITY, SUBCELLULAR LOCATION, AND INDUCTION AT 35 DEGREES
RP   CELSIUS.
RC   STRAIN=B31-4A; PLASMID=cp32-6;
RX   PubMed=10948116; DOI=10.1128/iai.68.9.4992-5001.2000;
RA   Porcella S.F., Fitzpatrick C.A., Bono J.L.;
RT   "Expression and immunological analysis of the plasmid-borne mlp genes of
RT   Borrelia burgdorferi strain B31.";
RL   Infect. Immun. 68:4992-5001(2000).
CC   -!- FUNCTION: An outer membrane protein that may participate in
CC       pathogenesis. Some human Lyme disease patients have antibodies against
CC       this protein (PubMed:10948116). The Mlp proteins probably undergo
CC       intragenic recombination, generating new alleles (Probable).
CC       {ECO:0000269|PubMed:10948116, ECO:0000305|PubMed:10948116}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000305|PubMed:10948116}; Lipid-anchor
CC       {ECO:0000305|PubMed:10948116}.
CC   -!- INDUCTION: Strongly induced when grown at 35 degrees Celsius.
CC       {ECO:0000269|PubMed:10948116}.
CC   -!- SIMILARITY: Belongs to the Multicopy lipoprotein (Mlp) family.
CC       {ECO:0000305}.
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DR   EMBL; AE001578; AAF07539.1; -; Genomic_DNA.
DR   RefSeq; NP_051319.1; NC_000951.1.
DR   RefSeq; WP_010883804.1; NC_000951.1.
DR   AlphaFoldDB; Q9S0B7; -.
DR   SMR; Q9S0B7; -.
DR   EnsemblBacteria; AAF07539; AAF07539; BB_M28.
DR   KEGG; bbu:BB_M28; -.
DR   PATRIC; fig|224326.49.peg.157; -.
DR   HOGENOM; CLU_134260_0_0_12; -.
DR   Proteomes; UP000001807; Plasmid cp32-6.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004983; Mlp.
DR   Pfam; PF03304; Mlp; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Plasmid;
KW   Reference proteome; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000305"
FT   CHAIN           18..149
FT                   /note="Lipoprotein MlpF"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_5004331819"
FT   REGION          26..58
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        33..58
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           18
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           18
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   149 AA;  16723 MW;  67CED7946F9B0ACC CRC64;
     MKIINILFCL FLLLLNSCNS NDNDTLKNNA QQTKSRGKRD LTQKEATPEK PKSKEELLRE
     KLSEDQKTHL DWLKEALGND GEFDKFLGYD ESKIKSALNH IKSELDKCTG DNSEQQKSTF
     KQTVQGFFSG GNIDNFANNA VSNCNNGGS