ID   MLRA_CHICK              Reviewed;         165 AA.
AC   P02610;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 2.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Myosin regulatory light chain 2A, cardiac muscle isoform;
DE            Short=G2;
DE            Short=MLC-2A;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2984206; DOI=10.1016/s0021-9258(18)89289-4;
RA   Winter B., Klapthor H., Wiebauer K., Delius H., Arnold H.H.;
RT   "Isolation and characterization of the chicken cardiac myosin light chain
RT   (L-2A) gene. Evidence for two additional N-terminal amino acids.";
RL   J. Biol. Chem. 260:4478-4483(1985).
RN   [2]
RP   PROTEIN SEQUENCE OF 3-165.
RX   PubMed=7319048; DOI=10.1016/0014-5793(81)80789-2;
RA   Matsuda G., Maita T., Kato Y., Chen J., Umegane T.;
RT   "Amino acid sequences of the cardiac L-2A, L-2B and gizzard 17 000-Mr light
RT   chains of chicken muscle myosin.";
RL   FEBS Lett. 135:232-236(1981).
CC   -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC   -!- PTM: The N-terminus is blocked. N,N,N-trimethylalanine, found in other
CC       myosin light chains would not have been detected in the N-terminal
CC       tryptic peptide in PubMed:7319048 because it would remain trimethylated
CC       and ninhydrin negative after hydrolysis.
CC   -!- MISCELLANEOUS: This chain binds calcium.
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DR   EMBL; M10990; AAA48975.1; -; Genomic_DNA.
DR   PIR; C03044; MOCHAC.
DR   AlphaFoldDB; P02610; -.
DR   SMR; P02610; -.
DR   STRING; 9031.ENSGALP00000034439; -.
DR   PaxDb; P02610; -.
DR   VEuPathDB; HostDB:geneid_416874; -.
DR   eggNOG; KOG0031; Eukaryota.
DR   PhylomeDB; P02610; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0030016; C:myofibril; IBA:GO_Central.
DR   GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR   GO; GO:0060048; P:cardiac muscle contraction; IBA:GO_Central.
DR   GO; GO:0007507; P:heart development; IBA:GO_Central.
DR   GO; GO:0042694; P:muscle cell fate specification; IBA:GO_Central.
DR   CDD; cd00051; EFh; 1.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF00036; EF-hand_1; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 3.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Methylation;
KW   Motor protein; Muscle protein; Myosin; Reference proteome; Repeat.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..165
FT                   /note="Myosin regulatory light chain 2A, cardiac muscle
FT                   isoform"
FT                   /id="PRO_0000019306"
FT   DOMAIN          24..59
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          94..128
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          129..164
FT                   /note="EF-hand 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         37
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         39
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         41
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         48
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   MOD_RES         2
FT                   /note="N,N,N-trimethylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:P02608"
SQ   SEQUENCE   165 AA;  18705 MW;  0FA147C597699B52 CRC64;
     MAPKKAKKRI EGANSNVFSM FEQAQIQEFK EAFTIMDQNR DGFIDKADLR DTFAALGRLN
     VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGLKSAYIKE
     MLMTQEGRFS QEEIDQMFAA FPPDVSGNLD YKNLVHVITH GEEKD