MLRA_HUMAN
ID MLRA_HUMAN Reviewed; 175 AA.
AC Q01449; B2R4L3;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Myosin regulatory light chain 2, atrial isoform;
DE Short=MLC-2a;
DE Short=MLC2a;
DE Short=Myosin light chain 2a;
DE AltName: Full=Myosin regulatory light chain 7;
GN Name=MYL7; Synonyms=MYL2A, MYLC2A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=1429676; DOI=10.1016/s0021-9258(18)50090-9;
RA Hailstones D.L., Barton P., Chan-Thomas P., Sutherland C.J., Hardeman E.C.,
RA Gunning P.W.;
RT "Differential regulation of the atrial isoforms of the myosin light chains
RT during striated muscle development.";
RL J. Biol. Chem. 267:23295-23300(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- INTERACTION:
CC Q01449; P59942: MCCD1; NbExp=3; IntAct=EBI-10222416, EBI-11987923;
CC Q01449; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-10222416, EBI-16439278;
CC Q01449; P78424: POU6F2; NbExp=3; IntAct=EBI-10222416, EBI-12029004;
CC Q01449; O75688-3: PPM1B; NbExp=3; IntAct=EBI-10222416, EBI-17715099;
CC Q01449; Q9UIG4: PSORS1C2; NbExp=3; IntAct=EBI-10222416, EBI-11974061;
CC Q01449; P51687: SUOX; NbExp=3; IntAct=EBI-10222416, EBI-3921347;
CC Q01449; O94842: TOX4; NbExp=3; IntAct=EBI-10222416, EBI-948613;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in adult atrial muscle.
CC {ECO:0000269|PubMed:1429676}.
CC -!- MISCELLANEOUS: This chain binds calcium.
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DR EMBL; M94547; AAA59889.1; -; mRNA.
DR EMBL; AK311869; BAG34810.1; -; mRNA.
DR EMBL; BC027915; AAH27915.1; -; mRNA.
DR CCDS; CCDS5478.1; -.
DR PIR; A44451; A44451.
DR RefSeq; NP_067046.1; NM_021223.2.
DR AlphaFoldDB; Q01449; -.
DR SMR; Q01449; -.
DR BioGRID; 121828; 8.
DR IntAct; Q01449; 7.
DR STRING; 9606.ENSP00000223364; -.
DR ChEMBL; CHEMBL3831286; -.
DR iPTMnet; Q01449; -.
DR PhosphoSitePlus; Q01449; -.
DR BioMuta; MYL7; -.
DR DMDM; 38604716; -.
DR jPOST; Q01449; -.
DR MassIVE; Q01449; -.
DR PaxDb; Q01449; -.
DR PeptideAtlas; Q01449; -.
DR PRIDE; Q01449; -.
DR ProteomicsDB; 57954; -.
DR Antibodypedia; 2833; 298 antibodies from 29 providers.
DR DNASU; 58498; -.
DR Ensembl; ENST00000223364.7; ENSP00000223364.3; ENSG00000106631.8.
DR GeneID; 58498; -.
DR KEGG; hsa:58498; -.
DR MANE-Select; ENST00000223364.7; ENSP00000223364.3; NM_021223.3; NP_067046.1.
DR UCSC; uc003tkg.4; human.
DR CTD; 58498; -.
DR DisGeNET; 58498; -.
DR GeneCards; MYL7; -.
DR HGNC; HGNC:21719; MYL7.
DR HPA; ENSG00000106631; Tissue enriched (heart).
DR MIM; 613993; gene.
DR neXtProt; NX_Q01449; -.
DR OpenTargets; ENSG00000106631; -.
DR PharmGKB; PA134939476; -.
DR VEuPathDB; HostDB:ENSG00000106631; -.
DR eggNOG; KOG0031; Eukaryota.
DR GeneTree; ENSGT00940000161682; -.
DR InParanoid; Q01449; -.
DR OMA; ALTHWGQ; -.
DR OrthoDB; 1435392at2759; -.
DR PhylomeDB; Q01449; -.
DR TreeFam; TF314218; -.
DR PathwayCommons; Q01449; -.
DR Reactome; R-HSA-445355; Smooth Muscle Contraction.
DR SignaLink; Q01449; -.
DR BioGRID-ORCS; 58498; 16 hits in 1069 CRISPR screens.
DR ChiTaRS; MYL7; human.
DR GeneWiki; MYL7; -.
DR GenomeRNAi; 58498; -.
DR Pharos; Q01449; Tbio.
DR PRO; PR:Q01449; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q01449; protein.
DR Bgee; ENSG00000106631; Expressed in cardiac muscle of right atrium and 97 other tissues.
DR ExpressionAtlas; Q01449; baseline and differential.
DR Genevisible; Q01449; HS.
DR GO; GO:0031672; C:A band; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0043197; C:dendritic spine; IEA:Ensembl.
DR GO; GO:0030016; C:myofibril; IBA:GO_Central.
DR GO; GO:0016459; C:myosin complex; NAS:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0048738; P:cardiac muscle tissue development; IBA:GO_Central.
DR GO; GO:0060047; P:heart contraction; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Acetylation; Calcium; Metal-binding; Motor protein; Muscle protein; Myosin;
KW Phosphoprotein; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:F1SSF9"
FT CHAIN 2..175
FT /note="Myosin regulatory light chain 2, atrial isoform"
FT /id="PRO_0000198725"
FT DOMAIN 32..67
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 102..137
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 138..173
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 47
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 49
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 56
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:F1SSF9"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9QVP4"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9QVP4"
SQ SEQUENCE 175 AA; 19448 MW; A21DBD518CAB90EB CRC64;
MASRKAGTRG KVAATKQAQR GSSNVFSMFE QAQIQEFKEA FSCIDQNRDG IICKADLRET
YSQLGKVSVP EEELDAMLQE GKGPINFTVF LTLFGEKLNG TDPEEAILSA FRMFDPSGKG
VVNKDEFKQL LLTQADKFSP AEVEQMFALT PMDLAGNIDY KSLCYIITHG DEKEE
