MLRA_MOUSE
ID MLRA_MOUSE Reviewed; 175 AA.
AC Q9QVP4; Q63977; Q9JIE8;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Myosin regulatory light chain 2, atrial isoform;
DE Short=MLC-2a;
DE Short=MLC2a;
DE Short=Myosin light chain 2a;
DE AltName: Full=Myosin regulatory light chain 7;
GN Name=Myl7; Synonyms=Mylc2a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Heart;
RX PubMed=8777429;
RA Palermo J., Gulick J., Ng W., Grupp I.L., Grupp G., Robbins J.;
RT "Remodeling the mammalian heart using transgenesis.";
RL Cell. Mol. Biol. Res. 41:501-509(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ; TISSUE=Liver;
RX PubMed=11124527; DOI=10.1159/000056782;
RA Doevendans P.A., Bronsaer R., Lozano P.R., Kubalak S., van Bilsen M.;
RT "The murine atrial myosin light chain-2 gene: a member of an evolutionarily
RT conserved family of contractile proteins.";
RL Cytogenet. Cell Genet. 90:248-252(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart, and Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 13-175, AND TISSUE SPECIFICITY.
RX PubMed=8207020; DOI=10.1016/s0021-9258(19)89483-8;
RA Kubalak S.W., Miller-Hance W.C., O'Brien T.X., Dyson E., Chien K.R.;
RT "Chamber specification of atrial myosin light chain-2 expression precedes
RT septation during murine cardiogenesis.";
RL J. Biol. Chem. 269:16961-16970(1994).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22 AND SER-23, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in adult atrial muscle.
CC {ECO:0000269|PubMed:8207020}.
CC -!- MISCELLANEOUS: This chain binds calcium.
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DR EMBL; AF232920; AAF76860.1; -; Genomic_DNA.
DR EMBL; BC061100; AAH61100.1; -; mRNA.
DR EMBL; S70785; AAB31016.1; -; mRNA.
DR CCDS; CCDS24408.1; -.
DR RefSeq; NP_075017.2; NM_022879.2.
DR AlphaFoldDB; Q9QVP4; -.
DR SMR; Q9QVP4; -.
DR IntAct; Q9QVP4; 4.
DR MINT; Q9QVP4; -.
DR STRING; 10090.ENSMUSP00000099985; -.
DR iPTMnet; Q9QVP4; -.
DR PhosphoSitePlus; Q9QVP4; -.
DR jPOST; Q9QVP4; -.
DR MaxQB; Q9QVP4; -.
DR PaxDb; Q9QVP4; -.
DR PRIDE; Q9QVP4; -.
DR ProteomicsDB; 295681; -.
DR Antibodypedia; 2833; 298 antibodies from 29 providers.
DR DNASU; 17898; -.
DR Ensembl; ENSMUST00000102921; ENSMUSP00000099985; ENSMUSG00000020469.
DR GeneID; 17898; -.
DR KEGG; mmu:17898; -.
DR UCSC; uc007hxl.1; mouse.
DR CTD; 58498; -.
DR MGI; MGI:107495; Myl7.
DR VEuPathDB; HostDB:ENSMUSG00000020469; -.
DR eggNOG; KOG0031; Eukaryota.
DR GeneTree; ENSGT00940000161682; -.
DR HOGENOM; CLU_061288_9_0_1; -.
DR InParanoid; Q9QVP4; -.
DR OMA; ALTHWGQ; -.
DR OrthoDB; 1435392at2759; -.
DR PhylomeDB; Q9QVP4; -.
DR TreeFam; TF314218; -.
DR Reactome; R-MMU-445355; Smooth Muscle Contraction.
DR BioGRID-ORCS; 17898; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Myl7; mouse.
DR PRO; PR:Q9QVP4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9QVP4; protein.
DR Bgee; ENSMUSG00000020469; Expressed in cardiac atrium and 89 other tissues.
DR ExpressionAtlas; Q9QVP4; baseline and differential.
DR Genevisible; Q9QVP4; MM.
DR GO; GO:0031672; C:A band; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0043197; C:dendritic spine; IDA:MGI.
DR GO; GO:0030016; C:myofibril; IBA:GO_Central.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0048738; P:cardiac muscle tissue development; IBA:GO_Central.
DR GO; GO:0060047; P:heart contraction; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Acetylation; Calcium; Metal-binding; Motor protein; Muscle protein; Myosin;
KW Phosphoprotein; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:F1SSF9"
FT CHAIN 2..175
FT /note="Myosin regulatory light chain 2, atrial isoform"
FT /id="PRO_0000198726"
FT DOMAIN 32..67
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 102..137
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 138..173
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 47
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 49
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 56
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:F1SSF9"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 10..11
FT /note="GK -> Q (in Ref. 2; AAF76860)"
FT /evidence="ECO:0000305"
FT CONFLICT 36..39
FT /note="EFKE -> DSRK (in Ref. 2; AAF76860)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="A -> T (in Ref. 4; AAB31016)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 175 AA; 19450 MW; 44C2D76C5D1D63CC CRC64;
MASRKAGTRG KAAATKQAQR GSSNVFSMFE QAQIQEFKEA FSCIDQNRDG IICKSDLKET
YSQLGRVSVP EEELDAMLQE GKGPINFTVF LTLFGEKLNG TDPEEAILSA FRMFDPSGQG
VVNKEEFKQL LMTQADKFSP AEVEQLFALT PMDLAGNIDY KSLCYIITHG DEKEE
