MLRA_PIG
ID MLRA_PIG Reviewed; 175 AA.
AC F1SSF9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2016, sequence version 3.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Myosin regulatory light chain 2, atrial isoform {ECO:0000250|UniProtKB:Q01449};
DE Short=MLC-2a {ECO:0000250|UniProtKB:Q01449};
DE Short=MLC2a {ECO:0000250|UniProtKB:Q01449};
DE Short=Myosin light chain 2a {ECO:0000250|UniProtKB:Q01449};
DE AltName: Full=Myosin regulatory light chain 7 {ECO:0000250|UniProtKB:Q01449};
GN Name=MYL7;
GN Synonyms=MYL2A {ECO:0000250|UniProtKB:Q01449},
GN MYLC2A {ECO:0000250|UniProtKB:Q01449};
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823 {ECO:0000312|Proteomes:UP000008227};
RN [1] {ECO:0000312|Proteomes:UP000008227}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Duroc;
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 2-175, ACETYLATION AT ALA-2, PHOSPHORYLATION AT SER-23,
RP MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RA Gregorich Z.R., Cai W., Chen A.J., Peng Y., Ge Y.;
RT "Distinct sequences and post-translational modifications in cardiac atrial
RT and ventricular myosin light chains revealed by top-down mass
RT spectrometry.";
RL Submitted (JUL-2016) to UniProtKB.
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC {ECO:0000305}.
CC -!- MISCELLANEOUS: This chain binds calcium. {ECO:0000305}.
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DR EMBL; CU928907; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003134933.1; XM_003134885.3.
DR AlphaFoldDB; F1SSF9; -.
DR SMR; F1SSF9; -.
DR STRING; 9823.ENSSSCP00000017742; -.
DR PaxDb; F1SSF9; -.
DR PeptideAtlas; F1SSF9; -.
DR Ensembl; ENSSSCT00000018234; ENSSSCP00000017742; ENSSSCG00000016751.
DR Ensembl; ENSSSCT00005030441; ENSSSCP00005018588; ENSSSCG00005018974.
DR Ensembl; ENSSSCT00025046763; ENSSSCP00025020043; ENSSSCG00025034032.
DR Ensembl; ENSSSCT00030049951; ENSSSCP00030022709; ENSSSCG00030035900.
DR Ensembl; ENSSSCT00035045850; ENSSSCP00035018314; ENSSSCG00035034564.
DR Ensembl; ENSSSCT00040083973; ENSSSCP00040036589; ENSSSCG00040061660.
DR Ensembl; ENSSSCT00045061544; ENSSSCP00045043244; ENSSSCG00045035371.
DR Ensembl; ENSSSCT00050046386; ENSSSCP00050019181; ENSSSCG00050034485.
DR Ensembl; ENSSSCT00055009958; ENSSSCP00055007885; ENSSSCG00055004963.
DR Ensembl; ENSSSCT00060023508; ENSSSCP00060009828; ENSSSCG00060017514.
DR Ensembl; ENSSSCT00065082397; ENSSSCP00065035899; ENSSSCG00065060071.
DR Ensembl; ENSSSCT00065082499; ENSSSCP00065035947; ENSSSCG00065060222.
DR GeneID; 100514437; -.
DR KEGG; ssc:100514437; -.
DR CTD; 58498; -.
DR eggNOG; KOG0031; Eukaryota.
DR GeneTree; ENSGT00950000182787; -.
DR HOGENOM; CLU_061288_9_3_1; -.
DR InParanoid; F1SSF9; -.
DR OMA; ALTHWGQ; -.
DR OrthoDB; 1435392at2759; -.
DR TreeFam; TF314218; -.
DR Proteomes; UP000008227; Chromosome 18.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000016751; Expressed in heart left ventricle and 38 other tissues.
DR ExpressionAtlas; F1SSF9; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030016; C:myofibril; IBA:GO_Central.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0048738; P:cardiac muscle tissue development; IBA:GO_Central.
DR GO; GO:0060047; P:heart contraction; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Acetylation; Calcium; Direct protein sequencing; Metal-binding;
KW Motor protein; Muscle protein; Myosin; Phosphoprotein; Reference proteome;
KW Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.2"
FT CHAIN 2..175
FT /note="Myosin regulatory light chain 2, atrial isoform"
FT /id="PRO_0000437966"
FT DOMAIN 32..67
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 102..137
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 138..173
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 47
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 49
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 56
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.1"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9QVP4"
FT MOD_RES 23
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|Ref.2"
SQ SEQUENCE 175 AA; 19474 MW; FD53878643FA8AB4 CRC64;
MASRKAGTRS KAAATKQAQR GSSNVFSMFE QAQIQEFKEA FSCIDQNRDG IICKSDLRET
YSQLGKVNVP EEELDAMLQE GKGPINFTVF LTLFGEKLNG TDPEEAILSA FRLFDPSGKG
VVNKDQFKQL LLTQADKFSP AEVEQMFALT PMDLAGNIDY KSLCYIITHG DEKEE
