MLR_DICDI
ID MLR_DICDI Reviewed; 161 AA.
AC P13833; Q552B5; Q75JL0;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Myosin regulatory light chain;
DE AltName: Full=RMLC;
GN Name=mlcR; Synonyms=mlcA; ORFNames=DDB_G0276077;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2550795; DOI=10.1128/mcb.9.7.3073-3080.1989;
RA Tafuri S.R., Rushforth A.M., Kuczmarski E.R., Chisholm R.L.;
RT "Dictyostelium discoideum myosin: isolation and characterization of cDNAs
RT encoding the regulatory light chain.";
RL Mol. Cell. Biol. 9:3073-3080(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains (two
CC regulatory light chains and two essential light chains).
CC -!- MISCELLANEOUS: The sequences of all three RMLC calcium-binding domains
CC have diverged significantly from that of calmodulin. Therefore, it
CC seems unlikely that these light chains bind calcium. It is potential
CC that phosphorylation has taken over the role of calcium-binding in the
CC regulation of Dictyostelium myosin.
CC -!- MISCELLANEOUS: In D.discoideum, cAMP stimulation induces RMLC
CC phosphorylation; this chain is phosphorylated on a serine residue.
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DR EMBL; M25251; AAA33226.1; -; mRNA.
DR EMBL; AAFI02000014; EAL69338.1; -; Genomic_DNA.
DR PIR; A32496; A30938.
DR RefSeq; XP_643313.1; XM_638221.1.
DR AlphaFoldDB; P13833; -.
DR SMR; P13833; -.
DR STRING; 44689.DDB0185146; -.
DR PaxDb; P13833; -.
DR EnsemblProtists; EAL69338; EAL69338; DDB_G0276077.
DR GeneID; 8620359; -.
DR KEGG; ddi:DDB_G0276077; -.
DR dictyBase; DDB_G0276077; mlcR.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_2_1_1; -.
DR InParanoid; P13833; -.
DR OMA; FPPDMCG; -.
DR PhylomeDB; P13833; -.
DR Reactome; R-DDI-5627123; RHO GTPases activate PAKs.
DR PRO; PR:P13833; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0042641; C:actomyosin; IDA:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016460; C:myosin II complex; IDA:dictyBase.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0140660; F:cytoskeletal motor activator activity; IDA:dictyBase.
DR GO; GO:0032036; F:myosin heavy chain binding; IDA:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0031034; P:myosin filament assembly; IDA:dictyBase.
DR GO; GO:0031038; P:myosin II filament organization; IDA:dictyBase.
DR GO; GO:1903610; P:regulation of calcium-dependent ATPase activity; IDA:dictyBase.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; HDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 2: Evidence at transcript level;
KW Motor protein; Myosin; Phosphoprotein; Reference proteome; Repeat.
FT CHAIN 1..161
FT /note="Myosin regulatory light chain"
FT /id="PRO_0000198759"
FT DOMAIN 20..55
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 56..91
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 93..128
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 13
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 14
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 161 AA; 18323 MW; 354B704B20F4E2C3 CRC64;
MASTKRRLNR EESSVVLGEE QVAELKEAFE LFDKDRTGFI KKDALKTTCK QFGVFVMEDQ
LDAMFAEADT TKSGAIGFPE FMSMMSRRMK QTSNEQILMN AFKTFDPEGN GYILTKDLSK
ALTTLGDKLT EAELQELLSI SENEQKQVKY DLFVNTLFSK K
