ID   MLR_LUMTE               Reviewed;         195 AA.
AC   P80164;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Myosin regulatory light chain, striated muscle, 25 kDa isoform;
DE            Short=LC25;
OS   Lumbricus terrestris (Common earthworm).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Oligochaeta; Crassiclitellata; Lumbricina; Lumbricidae; Lumbricinae;
OC   Lumbricus.
OX   NCBI_TaxID=6398;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=8425543; DOI=10.1111/j.1432-1033.1993.tb19903.x;
RA   Serwe M., Meyer H.E., Craig A.G., Carlhoff D., D'Haese J.;
RT   "Complete amino acid sequence of the regulatory light chain of obliquely
RT   striated muscle myosin from earthworm, Lumbricus terrestris.";
RL   Eur. J. Biochem. 211:341-346(1993).
CC   -!- FUNCTION: Plays an important role in regulation of muscle cell
CC       contractile activity.
CC   -!- SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains.
CC   -!- MISCELLANEOUS: This chain binds calcium.
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DR   PIR; S28845; S28845.
DR   AlphaFoldDB; P80164; -.
DR   SMR; P80164; -.
DR   GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13405; EF-hand_6; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   Calcium; Direct protein sequencing; Metal-binding; Motor protein;
KW   Muscle protein; Myosin; Repeat.
FT   CHAIN           1..195
FT                   /note="Myosin regulatory light chain, striated muscle, 25
FT                   kDa isoform"
FT                   /id="PRO_0000198753"
FT   DOMAIN          55..90
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          124..159
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          1..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..20
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         68
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         70
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         72
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         79
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   VARIANT         65
FT                   /note="T -> A (in 20% of the molecules)"
FT   VARIANT         70
FT                   /note="D -> N (in 20% of the molecules)"
FT   VARIANT         76..78
FT                   /note="GPD -> DES (in 20% of the molecules)"
FT   VARIANT         81..82
FT                   /note="GN -> AA (in 20% of the molecules)"
FT   VARIANT         84
FT                   /note="F -> Y (in 20% of the molecules)"
FT   VARIANT         127..128
FT                   /note="GT -> SS (in 20% of the molecules)"
FT   VARIANT         141
FT                   /note="L -> V (in 20% of the molecules)"
FT   VARIANT         168
FT                   /note="V -> L (in 20% of the molecules)"
SQ   SEQUENCE   195 AA;  21945 MW;  34BF85EB4D4E7F73 CRC64;
     AKDKEKKEKK DKKKDDAPAE EAPAAAAAPA EEAAPTPSAA PIHKVGNVFA LFKQNQIQEF
     KEAFTMIDQD RDGIIGPDDL GNIFQQIGRE VDPKVVKEML AESAEKLNFT HFLTLFGEKL
     HGTDTEGTLR DAFALFDEDK LGYLLEEYVK DLLTNVGDQY NKDEIKQVWK EAPIEGGKFD
     YVKFVRLIKR GKEEE