MLR_PHYPO
ID MLR_PHYPO Reviewed; 147 AA.
AC P08053;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Myosin regulatory light chain;
DE AltName: Full=Calcium-binding light chain;
OS Physarum polycephalum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Myxogastria;
OC Myxogastromycetidae; Physariida; Physaraceae; Physarum.
OX NCBI_TaxID=5791;
RN [1]
RP PROTEIN SEQUENCE, NUCLEOTIDE SEQUENCE OF 2-147, AND ACETYLATION AT THR-1.
RX PubMed=3335500; DOI=10.1016/s0021-9258(19)57393-8;
RA Kobayashi T., Takagi T., Konishi K., Hamada Y., Kawagushi M., Kohama K.;
RT "Amino acid sequence of the calcium-binding light chain of myosin from the
RT lower eukaryote, Physarum polycephalum.";
RL J. Biol. Chem. 263:305-313(1988).
CC -!- MISCELLANEOUS: This chain binds two moles of calcium.
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DR EMBL; J03499; AAA29978.1; -; mRNA.
DR PIR; A29910; A29910.
DR PDB; 2BL0; X-ray; 1.75 A; B=1-145.
DR PDBsum; 2BL0; -.
DR AlphaFoldDB; P08053; -.
DR SMR; P08053; -.
DR iPTMnet; P08053; -.
DR EvolutionaryTrace; P08053; -.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd00051; EFh; 1.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF00036; EF-hand_1; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Calcium; Direct protein sequencing;
KW Metal-binding; Motor protein; Myosin; Repeat.
FT CHAIN 1..147
FT /note="Myosin regulatory light chain"
FT /id="PRO_0000198758"
FT DOMAIN 2..37
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 73..108
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 109..144
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 15
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 17
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 26
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 86
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 90
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 92
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT BINDING 97
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000305"
FT MOD_RES 1
FT /note="N-acetylthreonine"
FT /evidence="ECO:0000269|PubMed:3335500"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 24..26
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 27..33
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 40..50
FT /evidence="ECO:0007829|PDB:2BL0"
FT STRAND 52..55
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 57..64
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 71..74
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 75..85
FT /evidence="ECO:0007829|PDB:2BL0"
FT STRAND 87..94
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 95..104
FT /evidence="ECO:0007829|PDB:2BL0"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 111..118
FT /evidence="ECO:0007829|PDB:2BL0"
FT STRAND 127..130
FT /evidence="ECO:0007829|PDB:2BL0"
FT HELIX 131..140
FT /evidence="ECO:0007829|PDB:2BL0"
SQ SEQUENCE 147 AA; 16081 MW; F1E5A1431912E896 CRC64;
TASADQIQEC FQIFDKDNDG KVSIEELGSA LRSLGKNPTN AELNTIKGQL NAKEFDLATF
KTVYRKPIKT PTEQSKEMLD AFRALDKEGN GTIQEAELRQ LLLNLGDALT SSEVEELMKE
VSVSGDGAIN YESFVDMLVT GYPLASA
