ID   MLR_TODPA               Reviewed;         153 AA.
AC   P08052;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Myosin regulatory light chain LC-2, mantle muscle;
DE            Short=RLC;
OS   Todarodes pacificus (Japanese flying squid) (Ommastrephes pacificus).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda;
OC   Coleoidea; Decapodiformes; Teuthida; Oegopsina; Ommastrephidae; Todarodes.
OX   NCBI_TaxID=6637;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3436964; DOI=10.1093/oxfordjournals.jbchem.a122153;
RA   Maita T., Tanaka H., Konno K., Matsuda G.;
RT   "Amino acid sequence of the regulatory light chain of squid mantle muscle
RT   myosin.";
RL   J. Biochem. 102:1151-1157(1987).
CC   -!- FUNCTION: In molluscan muscle, calcium regulation is associated with
CC       myosin rather than with actin. Muscle myosin contains two types of
CC       light chains: the catalytic light chain, essential for ATPase activity,
CC       and the regulatory light chain, a calcium-binding protein responsible
CC       for Ca(2+) dependent binding and Ca(2+) dependent Mg-ATPase activity.
CC   -!- MISCELLANEOUS: This chain binds calcium.
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DR   PIR; A41510; A41510.
DR   PDB; 3I5F; X-ray; 3.10 A; B=1-153.
DR   PDB; 3I5G; X-ray; 2.60 A; B=1-153.
DR   PDB; 3I5H; X-ray; 3.40 A; B=1-153.
DR   PDB; 3I5I; X-ray; 3.30 A; B=1-153.
DR   PDBsum; 3I5F; -.
DR   PDBsum; 3I5G; -.
DR   PDBsum; 3I5H; -.
DR   PDBsum; 3I5I; -.
DR   AlphaFoldDB; P08052; -.
DR   SMR; P08052; -.
DR   EvolutionaryTrace; P08052; -.
DR   GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR   InterPro; IPR002048; EF_hand_dom.
DR   Pfam; PF13405; EF-hand_6; 1.
DR   SMART; SM00054; EFh; 2.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS00018; EF_HAND_1; 1.
DR   PROSITE; PS50222; EF_HAND_2; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Calcium; Direct protein sequencing; Metal-binding;
KW   Motor protein; Muscle protein; Myosin; Repeat.
FT   CHAIN           1..153
FT                   /note="Myosin regulatory light chain LC-2, mantle muscle"
FT                   /id="PRO_0000198757"
FT   DOMAIN          13..48
FT                   /note="EF-hand 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   DOMAIN          82..117
FT                   /note="EF-hand 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         26
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         28
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         30
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   BINDING         37
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   MOD_RES         1
FT                   /note="Blocked amino end (Ala)"
FT   HELIX           12..25
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   TURN            30..32
FT                   /evidence="ECO:0007829|PDB:3I5F"
FT   HELIX           35..44
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   HELIX           51..59
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   HELIX           68..72
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   TURN            73..80
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   HELIX           84..92
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   STRAND          97..101
FT                   /evidence="ECO:0007829|PDB:3I5F"
FT   HELIX           104..112
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   STRAND          113..116
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   HELIX           120..127
FT                   /evidence="ECO:0007829|PDB:3I5G"
FT   HELIX           139..147
FT                   /evidence="ECO:0007829|PDB:3I5G"
SQ   SEQUENCE   153 AA;  17558 MW;  2FBE6E02F8FB9F4E CRC64;
     AEEAPRRVKL SQRQMQELKE AFTMIDQDRD GFIGMEDLKD MFSSLGRVPP DDELNAMLKE
     CPGQLNFTAF LTLFGEKVSG TDPEDALRNA FSMFDEDGQG FIPEDYLKDL LENMGDNFSK
     EEIKNVWKDA PLKNKQFNYN KMVDIKGKAE DED