ID   ML_THOGV                Reviewed;         304 AA.
AC   Q80A33;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   23-FEB-2022, entry version 39.
DE   RecName: Full=Protein ML;
DE            Short=ML;
GN   OrderedLocusNames=Segment 6;
OS   Thogoto virus (isolate SiAr 126) (Tho).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Insthoviricetes; Articulavirales; Orthomyxoviridae; Thogotovirus.
OX   NCBI_TaxID=126796;
OH   NCBI_TaxID=34610; Amblyomma variegatum (Tropical bont tick).
OH   NCBI_TaxID=10144; Cavia cutleri (Guinea pig).
OH   NCBI_TaxID=210652; Mungos mungo (Banded mongoose).
OH   NCBI_TaxID=34631; Rhipicephalus appendiculatus (Brown ear tick).
OH   NCBI_TaxID=6941; Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=12552016; DOI=10.1128/jvi.77.4.2747-2752.2003;
RA   Hagmaier K., Jennings S., Buse J., Weber F., Kochs G.;
RT   "Novel gene product of Thogoto virus segment 6 codes for an interferon
RT   antagonist.";
RL   J. Virol. 77:2747-2752(2003).
RN   [2]
RP   FUNCTION.
RX   PubMed=15582653; DOI=10.1016/j.virol.2004.10.015;
RA   Jennings S., Martinez-Sobrido L., Garcia-Sastre A., Weber F., Kochs G.;
RT   "Thogoto virus ML protein suppresses IRF3 function.";
RL   Virology 331:63-72(2005).
RN   [3]
RP   FUNCTION.
RX   PubMed=18768974; DOI=10.1128/jvi.01284-08;
RA   Vogt C., Preuss E., Mayer D., Weber F., Schwemmle M., Kochs G.;
RT   "The interferon antagonist ML protein of thogoto virus targets general
RT   transcription factor IIB.";
RL   J. Virol. 82:11446-11453(2008).
RN   [4]
RP   FUNCTION.
RX   PubMed=19812269; DOI=10.1099/vir.0.015172-0;
RA   Buettner N., Vogt C., Martinez-Sobrido L., Weber F., Waibler Z., Kochs G.;
RT   "Thogoto virus ML protein is a potent inhibitor of the interferon
RT   regulatory factor-7 transcription factor.";
RL   J. Gen. Virol. 91:220-227(2010).
CC   -!- FUNCTION: Blocks host IRF3 and IRF7, thereby inhibiting IFN-beta
CC       expression and activation of host antiviral state.
CC       {ECO:0000269|PubMed:15582653, ECO:0000269|PubMed:18768974,
CC       ECO:0000269|PubMed:19812269}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Protein ML;
CC         IsoId=Q80A33-1; Sequence=Displayed;
CC       Name=Matrix protein;
CC         IsoId=Q77D96-1; Sequence=External;
CC   -!- CAUTION: It has been suggested that ML could target host TFIIB, but
CC       this effect could be indirect. {ECO:0000305|PubMed:18768974}.
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DR   EMBL; AF527531; AAO49497.1; -; Genomic_RNA.
DR   RefSeq; YP_145805.1; NC_006504.1.
DR   PDB; 5I5N; X-ray; 1.42 A; A/B=3-151.
DR   PDB; 5I5O; X-ray; 2.68 A; A/B=3-151.
DR   PDBsum; 5I5N; -.
DR   PDBsum; 5I5O; -.
DR   SMR; Q80A33; -.
DR   IntAct; Q80A33; 5.
DR   GeneID; 5075736; -.
DR   KEGG; vg:5075736; -.
DR   Proteomes; UP000008973; Genome.
DR   GO; GO:0039548; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039557; P:suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF7 activity; IEA:UniProtKB-KW.
DR   InterPro; IPR035212; Mx/ML_C.
DR   Pfam; PF17536; Mx_ML; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host IRF3 by virus; Inhibition of host IRF7 by virus;
KW   Inhibition of host RLR pathway by virus; Reference proteome;
KW   Viral immunoevasion.
FT   CHAIN           1..304
FT                   /note="Protein ML"
FT                   /id="PRO_0000423167"
FT   HELIX           10..13
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           16..18
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           19..27
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           31..43
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           49..63
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           70..74
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           84..96
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           100..104
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           109..120
FT                   /evidence="ECO:0007829|PDB:5I5N"
FT   HELIX           129..138
FT                   /evidence="ECO:0007829|PDB:5I5N"
SQ   SEQUENCE   304 AA;  34463 MW;  4B0661F42B8C2E58 CRC64;
     MASNLPVRSF SEVCCAEARA AIIQMENNPD ETVCNRIWKI HRDLQSSDLT TTVQVMMVYR
     FISKRVPEGC FAILSGVNTG MYNPRELKRS YVQSLSSGTS CEFLRSLDKL AKNLLAVHVC
     SDVKMSLNKR QVIDFISGEE DPTLHTAEHL TSLALDDSPS AVVYSGWQQE AIKLHNTIRK
     IATMRPADCK AGKFYSDILS ACDQTKELLD AFDQGKLAYD RDVVLIGWMD EIIKIFSKPD
     YLEAKGVSYQ VLKNVSNKVA LLRESIWWVT ELDGREYLFF DESWYLHGMS AFSDGVPGYE
     DFIY