ID   MMAA1_MYCTO             Reviewed;         286 AA.
AC   P9WPB0; L0T754; P0A5Q0; P0C5C3; P72025; P94922; P96934;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Mycolic acid methyltransferase MmaA1;
DE            EC=2.1.1.-;
DE   AltName: Full=S-adenosylmethionine-dependent methyltransferase;
DE            Short=AdoMet-MT;
DE            Short=SAM-MT;
GN   Name=mmaA1; Synonyms=mma1; OrderedLocusNames=MT0673;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Involved in the conversion of a cis-olefin into a trans-
CC       olefin with concomitant introduction of an allylic methyl branch at the
CC       proximal position of the precursor to both the methoxy and ketomycolic
CC       acids. It directly affects the cis- to trans ratio and indirectly
CC       affects the keto to methoxy ratio (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Lipid metabolism; mycolic acid biosynthesis.
CC   -!- SIMILARITY: Belongs to the CFA/CMAS family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK44899.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK44899.1; ALT_INIT; Genomic_DNA.
DR   PIR; B70614; B70614.
DR   RefSeq; WP_003403310.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WPB0; -.
DR   SMR; P9WPB0; -.
DR   EnsemblBacteria; AAK44899; AAK44899; MT0673.
DR   GeneID; 45424605; -.
DR   KEGG; mtc:MT0673; -.
DR   PATRIC; fig|83331.31.peg.716; -.
DR   HOGENOM; CLU_026434_3_0_11; -.
DR   UniPathway; UPA00915; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR003333; Mycolic_cyclopropane_synthase.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PIRSF; PIRSF003085; CMAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..286
FT                   /note="Mycolic acid methyltransferase MmaA1"
FT                   /id="PRO_0000426983"
FT   ACT_SITE        268
FT                   /evidence="ECO:0000250"
FT   BINDING         32..33
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..73
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         93..98
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
FT   BINDING         122..123
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   286 AA;  33149 MW;  229EB252DCA8C552 CRC64;
     MAKLRPYYEE SQSAYDISDD FFALFLDPTW VYTCAYFERD DMTLEEAQLA KVDLALDKLN
     LEPGMTLLDV GCGWGGALVR AVEKYDVNVI GLTLSRNHYE RSKDRLAAIG TQRRAEARLQ
     GWEEFEENVD RIVSFEAFDA FKKERYLTFF ERSYDILPDD GRMLLHSLFT YDRRWLHEQG
     IALTMSDLRF LKFLRESIFP GGELPSEPDI VDNAQAAGFT IEHVQLLQQH YARTLDAWAA
     NLQAARERAI AVQSEEVYNN FMHYLTGCAE RFRRGLINVA QFTMTK