MMDB_PROMO
ID MMDB_PROMO Reviewed; 395 AA.
AC O54031;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Methylmalonyl-CoA decarboxylase subunit beta {ECO:0000305};
DE EC=7.2.4.3 {ECO:0000269|PubMed:9428714};
GN Name=mmdB {ECO:0000303|PubMed:9428714};
OS Propionigenium modestum.
OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Propionigenium.
OX NCBI_TaxID=2333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=DSM 2376 / Gra Succ2;
RX PubMed=9428714; DOI=10.1111/j.1432-1033.1997.0590a.x;
RA Bott M., Pfister K., Burda P., Kalbermatter O., Woehlke G., Dimroth P.;
RT "Methylmalonyl-CoA decarboxylase from Propionigenium modestum--cloning and
RT sequencing of the structural genes and purification of the enzyme
RT complex.";
RL Eur. J. Biochem. 250:590-599(1997).
CC -!- FUNCTION: Tunnel subunit of the sodium ion pump methylmalonyl-CoA
CC decarboxylase, which converts the chemical energy of a decarboxylation
CC reaction into an electrochemical gradient of Na(+) ions across the
CC cytoplasmic membrane, thereby creating a sodium ion motive force that
CC is used for ATP synthesis (PubMed:9428714). The beta subunit catalyzes
CC the decarboxylation of the carboxybiotin carrier protein and the
CC coupled export of Na(+) ions (By similarity).
CC {ECO:0000250|UniProtKB:Q57286, ECO:0000269|PubMed:9428714}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-methylmalonyl-CoA + H(+)(out) + Na(+)(in) = CO2 +
CC Na(+)(out) + propanoyl-CoA; Xref=Rhea:RHEA:21396, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:29101, ChEBI:CHEBI:57327,
CC ChEBI:CHEBI:57392; EC=7.2.4.3; Evidence={ECO:0000269|PubMed:9428714};
CC -!- SUBUNIT: The methylmalonyl-CoA decarboxylase is composed of four
CC subunits: the carboxyltransferase alpha subunit (MmdA), the tunnel beta
CC subunit (MmdB), the biotin-containing gamma subunit (MmdC) and the
CC delta subunit (MmdD). {ECO:0000269|PubMed:9428714}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:9428714};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:9428714}.
CC -!- SIMILARITY: Belongs to the GcdB/MmdB/OadB family. {ECO:0000305}.
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DR EMBL; AJ002015; CAA05140.1; -; Genomic_DNA.
DR PIR; T44985; T44985.
DR SMR; O54031; -.
DR KEGG; ag:CAA05140; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004492; F:methylmalonyl-CoA decarboxylase activity; IEA:RHEA.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR005661; OadB_MmdB.
DR PANTHER; PTHR35806; PTHR35806; 1.
DR Pfam; PF03977; OAD_beta; 1.
DR PIRSF; PIRSF015658; MmdB_OadB; 1.
DR TIGRFAMs; TIGR01109; Na_pump_decarbB; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Sodium; Sodium transport;
KW Translocase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..395
FT /note="Methylmalonyl-CoA decarboxylase subunit beta"
FT /id="PRO_0000453536"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 395 AA; 41232 MW; CFA30A8C17D3C69C CRC64;
MLQAILDFYH STGFYGLNMG SIIMMLVACV FLYLAIAKEF EPLLLVPISF GILLTNLPFA
GMMAEPLLEV HEKLSASGAH LYTAHTAEPG GLLYYLFQGD HLGIFPPLIF LGVGAMTDFG
PLISNPKSLL LGAAAQFGIF VTFFGAIASG LFTAQEAASI GIIGGADGPT AIFLSSKLAP
HLMGPIAVAA YSYMALVPII QPPIMTALTS ETERKIKMSQ LRLVSKREKI IFPIVVTILV
SLIVPPAATL VGMLMLGNLF RECGVVGRLE DTAKNALINI ITIFLGVTVG ATATAEAFLK
VETLAILGLG IVAFGIGTGS GVLLAKFMNK LSKEPINPLL GSAGVSAVPM AARVSQVVGQ
KADPTNFLLM HAMGPNVAGV IGSAVSAGVL LSLFG