MME1_DROME
ID MME1_DROME Reviewed; 299 AA.
AC Q9VM51;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Mitochondrial magnesium exporter 1 {ECO:0000303|PubMed:26462626};
GN Name=MME1 {ECO:0000303|PubMed:26462626};
GN ORFNames=CG3476 {ECO:0000312|FlyBase:FBgn0031881};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AAL39736.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|EMBL:AAL39736.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL39736.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL39736.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=26462626; DOI=10.1016/j.bbamcr.2015.10.004;
RA Cui Y., Zhao S., Wang X., Zhou B.;
RT "A novel Drosophila mitochondrial carrier protein acts as a Mg(2+) exporter
RT in fine-tuning mitochondrial Mg(2+) homeostasis.";
RL Biochim. Biophys. Acta 1863:30-39(2016).
CC -!- FUNCTION: Mediates efflux of magnesium ions from mitochondria,
CC suggesting a role in magnesium homeostasis.
CC {ECO:0000269|PubMed:26462626}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane
CC {ECO:0000269|PubMed:26462626}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in accumulation
CC of magnesium ions in mitochondria, and reduced lifespan. A magnesium-
CC deficient diet rescues the reduced lifespan phenotype.
CC {ECO:0000269|PubMed:26462626}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000255|RuleBase:RU000488, ECO:0000305}.
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DR EMBL; AE014134; AAF52476.1; -; Genomic_DNA.
DR EMBL; AY069591; AAL39736.1; -; mRNA.
DR RefSeq; NP_609093.1; NM_135249.3.
DR AlphaFoldDB; Q9VM51; -.
DR SMR; Q9VM51; -.
DR IntAct; Q9VM51; 4.
DR STRING; 7227.FBpp0079017; -.
DR TCDB; 2.A.29.8.15; the mitochondrial carrier (mc) family.
DR PaxDb; Q9VM51; -.
DR PRIDE; Q9VM51; -.
DR DNASU; 33987; -.
DR EnsemblMetazoa; FBtr0079389; FBpp0079017; FBgn0031881.
DR GeneID; 33987; -.
DR KEGG; dme:Dmel_CG3476; -.
DR UCSC; CG3476-RA; d. melanogaster.
DR CTD; 33987; -.
DR FlyBase; FBgn0031881; MME1.
DR VEuPathDB; VectorBase:FBgn0031881; -.
DR eggNOG; KOG0758; Eukaryota.
DR GeneTree; ENSGT00940000167609; -.
DR HOGENOM; CLU_015166_16_0_1; -.
DR InParanoid; Q9VM51; -.
DR OMA; GVGGMCN; -.
DR OrthoDB; 1072378at2759; -.
DR PhylomeDB; Q9VM51; -.
DR BioGRID-ORCS; 33987; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33987; -.
DR PRO; PR:Q9VM51; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031881; Expressed in adult midgut (Drosophila) and 31 other tissues.
DR ExpressionAtlas; Q9VM51; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005740; C:mitochondrial envelope; IBA:GO_Central.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015227; F:acyl carnitine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015095; F:magnesium ion transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1902603; P:carnitine transmembrane transport; IBA:GO_Central.
DR GO; GO:0010961; P:cellular magnesium ion homeostasis; IDA:FlyBase.
DR GO; GO:1990616; P:magnesium ion export from mitochondrion; IDA:FlyBase.
DR GO; GO:1903830; P:magnesium ion transmembrane transport; IDA:FlyBase.
DR GO; GO:0006839; P:mitochondrial transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 2.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Magnesium; Membrane; Mitochondrion; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..299
FT /note="Mitochondrial magnesium exporter 1"
FT /id="PRO_0000439277"
FT TRANSMEM 79..99
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT REPEAT 12..103
FT /note="Solcar 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 112..200
FT /note="Solcar 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
FT REPEAT 210..296
FT /note="Solcar 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00282"
SQ SEQUENCE 299 AA; 32396 MW; EE0B76D9D0D96F26 CRC64;
MEEVEISTEK KSNPVKSFIA GGVGGMCNVL VGHPLDTIKV RLQTMPTPPP GQPPRYKGVI
DCAARTFRYE GFRGFYRGIS APLVGVTPIY AVDFAVYAAG KRLFQTDDHI RLTYPQIFAA
GALAGVCSAL VTVPTDRIKV LLQTQTVSNG PLLYNGTIDT AAKLYRQGGI RSLFKGTCAC
ILRDSPTGFY FVTYEFLQEL ARKKSANGKI STTSTILSGG TAGIVFWTLA VPFDVLKSRL
QSAPEGTYKH GIRSVFRNLM ATEGPKALFR GILPILLRAF PSTAAVFFGV ELTNDLLKA
