MMOB_METCA
ID MMOB_METCA Reviewed; 141 AA.
AC P18797; Q609N6; Q9LCT7;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Methane monooxygenase regulatory protein B;
GN Name=mmoB; OrderedLocusNames=MCA1196;
OS Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylococcus.
OX NCBI_TaxID=243233;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX PubMed=2505721; DOI=10.1007/bf00456094;
RA Stainthorpe A.C., Murrell J.C., Salmond G.P.C., Dalton H., Lees V.;
RT "Molecular analysis of methane monooxygenase from Methylococcus capsulatus
RT (Bath).";
RL Arch. Microbiol. 152:154-159(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA McDonald I., Murrell J.C.;
RL Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT "Genomic insights into methanotrophy: the complete genome sequence of
RT Methylococcus capsulatus (Bath).";
RL PLoS Biol. 2:1616-1628(2004).
RN [4]
RP IDENTIFICATION OF PROTEIN, AND PROTEIN SEQUENCE OF 3-19.
RA Pilkington S.J., Salmond G.P.C., Murrell J.C., Dalton H.;
RT "Identification of the gene encoding the regulatory protein B of soluble
RT methane monooxygenase.";
RL FEMS Microbiol. Lett. 72:345-348(1990).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=10393915; DOI=10.1073/pnas.96.14.7877;
RA Walters K.J., Gassner G.T., Lippard S.J., Wagner G.;
RT "Structure of the soluble methane monooxygenase regulatory protein B.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:7877-7882(1999).
RN [6]
RP CIRCULAR DICHROISM ANALYSIS, AND 3D-STRUCTURE MODELING.
RX PubMed=10381404; DOI=10.1016/s1074-5521(99)80062-3;
RA Brandstetter H., Whittington D.A., Lippard S.J., Frederick C.A.;
RT "Mutational and structural analyses of the regulatory protein B of soluble
RT methane monooxygenase from Methylococcus capsulatus (Bath).";
RL Chem. Biol. 6:441-449(1999).
CC -!- FUNCTION: The B protein acts as a regulator of electron flow through
CC the soluble mmo complex, switching the enzyme from an oxidase to a
CC hydroxylase in the presence of the substrate.
CC -!- SUBUNIT: The soluble methane monooxygenase (sMMO) consists of four
CC components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ),
CC B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).
CC -!- SIMILARITY: Belongs to the TmoD/XamoD family. {ECO:0000305}.
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DR EMBL; M90050; AAF04158.2; -; Genomic_DNA.
DR EMBL; AE017282; AAU92726.1; -; Genomic_DNA.
DR PIR; JL0103; JL0103.
DR RefSeq; WP_010960484.1; NC_002977.6.
DR PDB; 1CKV; NMR; -; A=1-141.
DR PDB; 4GAM; X-ray; 2.90 A; D/I/N/S=1-141.
DR PDBsum; 1CKV; -.
DR PDBsum; 4GAM; -.
DR AlphaFoldDB; P18797; -.
DR BMRB; P18797; -.
DR SMR; P18797; -.
DR DIP; DIP-60130N; -.
DR IntAct; P18797; 1.
DR STRING; 243233.MCA1196; -.
DR EnsemblBacteria; AAU92726; AAU92726; MCA1196.
DR KEGG; mca:MCA1196; -.
DR eggNOG; ENOG502ZPSX; Bacteria.
DR HOGENOM; CLU_1823101_0_0_6; -.
DR OMA; RAYTLGN; -.
DR OrthoDB; 1818784at2; -.
DR BioCyc; MetaCyc:MON-3865; -.
DR BRENDA; 1.14.13.25; 3305.
DR EvolutionaryTrace; P18797; -.
DR Proteomes; UP000006821; Chromosome.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro.
DR Gene3D; 3.90.56.10; -; 1.
DR InterPro; IPR003454; MOase_MmoB_DmpM.
DR InterPro; IPR036889; mOase_MmoB_DmpM_sf.
DR Pfam; PF02406; MmoB_DmpM; 1.
DR SUPFAM; SSF56029; SSF56029; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..141
FT /note="Methane monooxygenase regulatory protein B"
FT /id="PRO_0000096507"
FT CONFLICT 12
FT /note="I -> F (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:4GAM"
FT TURN 11..14
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 18..25
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 28..30
FT /evidence="ECO:0007829|PDB:4GAM"
FT STRAND 37..43
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 46..54
FT /evidence="ECO:0007829|PDB:4GAM"
FT TURN 55..58
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 59..63
FT /evidence="ECO:0007829|PDB:4GAM"
FT STRAND 69..72
FT /evidence="ECO:0007829|PDB:4GAM"
FT STRAND 74..88
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 89..96
FT /evidence="ECO:0007829|PDB:4GAM"
FT HELIX 102..105
FT /evidence="ECO:0007829|PDB:4GAM"
FT STRAND 108..128
FT /evidence="ECO:0007829|PDB:4GAM"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:4GAM"
SQ SEQUENCE 141 AA; 15984 MW; 86A1496846C5840F CRC64;
MSVNSNAYDA GIMGLKGKDF ADQFFADENQ VVHESDTVVL VLKKSDEINT FIEEILLTDY
KKNVNPTVNV EDRAGYWWIK ANGKIEVDCD EISELLGRQF NVYDFLVDVS STIGRAYTLG
NKFTITSELM GLDRKLEDYH A
