MMPLB_MYCS2
ID MMPLB_MYCS2 Reviewed; 954 AA.
AC A0QP18;
DT 29-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Mycolic acid-containing lipids exporter MmpL11 {ECO:0000305};
GN Name=mmpL11 {ECO:0000303|PubMed:23836904};
GN OrderedLocusNames=MSMEG_0241 {ECO:0000312|EMBL:ABK71905.1},
GN MSMEI_0234 {ECO:0000312|EMBL:AFP36715.1};
GN ORFNames=LJ00_01210 {ECO:0000312|EMBL:AIU05520.1};
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=25657281; DOI=10.1128/genomea.01520-14;
RA Mohan A., Padiadpu J., Baloni P., Chandra N.;
RT "Complete genome sequences of a Mycobacterium smegmatis laboratory strain
RT (MC2 155) and isoniazid-resistant (4XR1/R2) mutant strains.";
RL Genome Announc. 3:E01520-E01520(2015).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=23836904; DOI=10.1074/jbc.m113.473371;
RA Pacheco S.A., Hsu F.F., Powers K.M., Purdy G.E.;
RT "MmpL11 protein transports mycolic acid-containing lipids to the
RT mycobacterial cell wall and contributes to biofilm formation in
RT Mycobacterium smegmatis.";
RL J. Biol. Chem. 288:24213-24222(2013).
CC -!- FUNCTION: Contributes to cell wall biosynthesis and biofilm formation.
CC Transports the mycolic acid-containing lipids monomeromycolyl
CC diacylglycerol (MMDAG) and mycolate ester wax (WE) to the bacterial
CC surface. {ECO:0000269|PubMed:23836904}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:23836904}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Mutant has altered cell wall lipid composition,
CC reduced membrane permeability and altered biofilm formation. It
CC accumulates the mycolic acid-containing precursor mycolyl phospholipid
CC (MycPL) and fails to export MMDAG and mycolate ester wax to the
CC bacterial surface. Does not affect trehalose monomycolate (TMM)
CC transport. {ECO:0000269|PubMed:23836904}.
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DR EMBL; CP000480; ABK71905.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP36715.1; -; Genomic_DNA.
DR EMBL; CP009494; AIU05520.1; -; Genomic_DNA.
DR RefSeq; WP_011726771.1; NZ_SIJM01000065.1.
DR RefSeq; YP_884656.1; NC_008596.1.
DR AlphaFoldDB; A0QP18; -.
DR SMR; A0QP18; -.
DR STRING; 246196.MSMEI_0234; -.
DR PRIDE; A0QP18; -.
DR EnsemblBacteria; ABK71905; ABK71905; MSMEG_0241.
DR EnsemblBacteria; AFP36715; AFP36715; MSMEI_0234.
DR GeneID; 66738429; -.
DR KEGG; msb:LJ00_01210; -.
DR KEGG; msg:MSMEI_0234; -.
DR KEGG; msm:MSMEG_0241; -.
DR PATRIC; fig|246196.19.peg.237; -.
DR eggNOG; COG2409; Bacteria.
DR HOGENOM; CLU_005108_2_0_11; -.
DR OMA; HWSRRPE; -.
DR OrthoDB; 149255at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR InterPro; IPR004869; MMPL_dom.
DR InterPro; IPR000731; SSD.
DR Pfam; PF03176; MMPL; 2.
DR PROSITE; PS50156; SSD; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Cell wall biogenesis/degradation;
KW Lipid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..954
FT /note="Mycolic acid-containing lipids exporter MmpL11"
FT /id="PRO_0000432885"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 597..617
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 648..668
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 670..690
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 954 AA; 102692 MW; 2D9AF70EFB4B3571 CRC64;
MMRLSSTLRR FRWAVFATWL LLLVPSIYLA LNQSSNLTGG GFEVEGSQSL HVQRQLEEHF
PDQGASPLAL VAAPRADASY EDMNAAVVHL EKLAAEVPSV KIVPNPQQPA PQPDRPYVIT
LQLDFNNTGA VDVAKQLRQK VGIHGEEPGE SQNGKVKFYV IGQGALGAAA TQATKHDIAA
AEKWNMPIVL IVLLAVFGSL AAAALPLVLG VCTVVVTMGL VYLLSMFTTM SVFVTSTVSM
FGIAVAIDYS LFILMRFREE LRAGRDQQDA IDAAMATSGL AVALSGLTVI ASVTGIYLIN
TPVLVSMATG AILAVAVAVL TSTTLTPAVL ATFGKAAAKR SSYLHWSRRA EAAQSRFWTR
WTGAVMRRPW ASAIAAAILL LVLAAPAFNM VLGNSMQRQF DPTHEIRGGV NAAADALGPG
ALGPIRVLVT FPGEGDASSQ AATTTIEAVR QQMTKAPSVV SVQPPVVSDD NDSALLSAVL
SVDPEDMAAR EAIDWMRAEL PGVAGQNATI DVGGPTALIK DFDDRVSATQ PLVFVFVALI
AFVMLLVSIR SVVLAFKGVL MTVLSVAAAY GSLVVVFQWG WLEQLGFPRI SSLDSTIPPL
VLAMTFGLSM DYEIFLLTRI RERFLQTNST RDAVAYGVST SARTITSAAL IMIAVFIGFA
FAGMPLVAQL GVACAVAIAV DATVVRLVLV PALMAMFDQW NWWLPRWLDK ILPEVDFEKP
LPKIEVTDLV IIPDNIAALG PSGSDLRTMV RTAARMKTLA PQTISVADPL AFSGCTRPTT
RLSTQRAGRP KAHTPGLHPV TMWRGRLSVA VDALQTEADT EQAPVERRGP VETTNVQLPT
GDRLQIPTGA ETLRLAGYLI MCRNTTKDFE DFARLVDLMD SHTAALVLAS MDRYYCGRDP
SNRWVATQLV RRLADPQPSD EHDVRMSGPD AAEDWEKVRQ RCLSVAVAML EEAK
