MMPS4_MYCTU
ID MMPS4_MYCTU Reviewed; 140 AA.
AC P9WJS9; L0T6P9; O53736; P0A5K2;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 36.
DE RecName: Full=Siderophore export accessory protein MmpS4 {ECO:0000305};
DE AltName: Full=PGB14T-X;
GN Name=mmpS4; OrderedLocusNames=Rv0451c; ORFNames=MTV037.15c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9639933; DOI=10.1099/00221287-144-6-1619;
RA Chubb A.J., Woodman Z.L., da Silva Tatley F.M.P.R., Hoffmann H.J.,
RA Scholle R.R., Ehlers M.R.W.;
RT "Identification of Mycobacterium tuberculosis signal sequences that direct
RT the export of a leaderless beta-lactamase gene product in Escherichia
RT coli.";
RL Microbiology 144:1619-1629(1998).
RN [3]
RP INDUCTION.
RC STRAIN=H37Rv;
RX PubMed=12065475; DOI=10.1128/iai.70.7.3371-3381.2002;
RA Rodriguez G.M., Voskuil M.I., Gold B., Schoolnik G.K., Smith I.;
RT "IdeR, an essential gene in Mycobacterium tuberculosis: role of IdeR in
RT iron-dependent gene expression, iron metabolism, and oxidative stress
RT response.";
RL Infect. Immun. 70:3371-3381(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [5]
RP INDUCTION.
RC STRAIN=H37Rv;
RX PubMed=24737322; DOI=10.1074/jbc.m113.538959;
RA Radhakrishnan A., Kumar N., Wright C.C., Chou T.H., Tringides M.L.,
RA Bolla J.R., Lei H.T., Rajashankar K.R., Su C.C., Purdy G.E., Yu E.W.;
RT "Crystal structure of the transcriptional regulator Rv0678 of Mycobacterium
RT tuberculosis.";
RL J. Biol. Chem. 289:16526-16540(2014).
RN [6]
RP STRUCTURE BY NMR OF 52-140, FUNCTION, INTERACTION WITH MMPL4 AND MMPL5,
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=H37Rv;
RX PubMed=23431276; DOI=10.1371/journal.ppat.1003120;
RA Wells R.M., Jones C.M., Xi Z., Speer A., Danilchanka O., Doornbos K.S.,
RA Sun P., Wu F., Tian C., Niederweis M.;
RT "Discovery of a siderophore export system essential for virulence of
RT Mycobacterium tuberculosis.";
RL PLoS Pathog. 9:E1003120-E1003120(2013).
CC -!- FUNCTION: Part of an export system, which is required for biosynthesis
CC and secretion of siderophores. Essential for virulence.
CC {ECO:0000269|PubMed:23431276}.
CC -!- SUBUNIT: Interacts with MmpL4. Can also interact with MmpL5.
CC {ECO:0000269|PubMed:23431276}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:23431276}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Transcriptionally regulated by MmpR5 (PubMed:24737322).
CC Repressed by iron and IdeR (PubMed:12065475).
CC {ECO:0000269|PubMed:12065475, ECO:0000269|PubMed:24737322}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant does not exhibit a low iron
CC growth phenotype, but has attenuated virulence compared to the wild-
CC type strain. Deletion of both mmpS4 and mmpS5 drastically decreases
CC synthesis and secretion of siderophores, and greatly reduces virulence
CC in mice. {ECO:0000269|PubMed:23431276}.
CC -!- SIMILARITY: Belongs to the MmpS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC26395.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL123456; CCP43182.1; -; Genomic_DNA.
DR EMBL; AF017100; AAC26395.1; ALT_INIT; Genomic_DNA.
DR PIR; D70831; D70831.
DR RefSeq; NP_214965.1; NC_000962.3.
DR RefSeq; WP_003402272.1; NZ_NVQJ01000002.1.
DR PDB; 2LW3; NMR; -; A=52-140.
DR PDBsum; 2LW3; -.
DR AlphaFoldDB; P9WJS9; -.
DR BMRB; P9WJS9; -.
DR SMR; P9WJS9; -.
DR STRING; 83332.Rv0451c; -.
DR PaxDb; P9WJS9; -.
DR DNASU; 886321; -.
DR GeneID; 45424412; -.
DR GeneID; 886321; -.
DR KEGG; mtu:Rv0451c; -.
DR TubercuList; Rv0451c; -.
DR eggNOG; ENOG50329VV; Bacteria.
DR OMA; RLTYEVF; -.
DR PhylomeDB; P9WJS9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.2880; -; 1.
DR InterPro; IPR008693; MmpS.
DR InterPro; IPR038468; MmpS_C.
DR Pfam; PF05423; Mycobact_memb; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..140
FT /note="Siderophore export accessory protein MmpS4"
FT /id="PRO_0000216158"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:2LW3"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:2LW3"
FT STRAND 133..135
FT /evidence="ECO:0007829|PDB:2LW3"
SQ SEQUENCE 140 AA; 15404 MW; 5C94F52A73BEFD53 CRC64;
MLMRTWIPLV ILVVVIVGGF TVHRIRGFFG SENRPSYSDT NLENSKPFNP KHLTYEIFGP
PGTVADISYF DVNSEPQRVD GAVLPWSLHI TTNDAAVMGN IVAQGNSDSI GCRITVDGKV
RAERVSNEVN AYTYCLVKSA
