ID   MMR_MYCTU               Reviewed;         107 AA.
AC   P9WGF1; L0TE91; P69926; P95094;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Multidrug resistance protein Mmr;
GN   Name=mmr; OrderedLocusNames=Rv3065; ORFNames=MTCY22D7.17c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND INHIBITION BY CCCP.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9811672; DOI=10.1128/jb.180.22.6068-6071.1998;
RA   De Rossi E., Branzoni M., Cantoni R., Milano A., Riccardi G., Ciferri O.;
RT   "mmr, a Mycobacterium tuberculosis gene conferring resistance to small
RT   cationic dyes and inhibitors.";
RL   J. Bacteriol. 180:6068-6071(1998).
RN   [3]
RP   FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RX   PubMed=11574548; DOI=10.1074/jbc.m108231200;
RA   Ninio S., Rotem D., Schuldiner S.;
RT   "Functional analysis of novel multidrug transporters from human
RT   pathogens.";
RL   J. Biol. Chem. 276:48250-48256(2001).
CC   -!- FUNCTION: Multidrug efflux pump. Confers resistance to
CC       tetraphenylphosphonium (TPP), erythromycin, ethidium bromide,
CC       acriflavine, safranin O, pyronin Y and methyl viologen.
CC       {ECO:0000269|PubMed:11574548, ECO:0000269|PubMed:9811672}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=943 uM for methyl viologen {ECO:0000269|PubMed:11574548};
CC         Vmax=22085 nmol/min/mg enzyme with methyl viologen as substrate
CC         {ECO:0000269|PubMed:11574548};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11574548};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11574548}.
CC   -!- MISCELLANEOUS: Extrusion of TPP is inhibited by CCCP.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family. Mmr
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45874.1; -; Genomic_DNA.
DR   PIR; B70650; B70650.
DR   RefSeq; WP_003415995.1; NZ_NVQJ01000011.1.
DR   RefSeq; YP_177922.1; NC_000962.3.
DR   AlphaFoldDB; P9WGF1; -.
DR   SMR; P9WGF1; -.
DR   STRING; 83332.Rv3065; -.
DR   PaxDb; P9WGF1; -.
DR   DNASU; 887550; -.
DR   GeneID; 45427058; -.
DR   GeneID; 887550; -.
DR   KEGG; mtu:Rv3065; -.
DR   TubercuList; Rv3065; -.
DR   eggNOG; COG2076; Bacteria.
DR   OMA; ATTAMKY; -.
DR   PhylomeDB; P9WGF1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IMP:MTBBASE.
DR   GO; GO:0022857; F:transmembrane transporter activity; IDA:MTBBASE.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IDA:MTBBASE.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..107
FT                   /note="Multidrug resistance protein Mmr"
FT                   /id="PRO_0000108120"
FT   TRANSMEM        2..19
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   107 AA;  11083 MW;  3D372AC2FAE89834 CRC64;
     MIYLYLLCAI FAEVVATSLL KSTEGFTRLW PTVGCLVGYG IAFALLALSI SHGMQTDVAY
     ALWSAIGTAA IVLVAVLFLG SPISVMKVVG VGLIVVGVVT LNLAGAH