ID   MMS48_MAGGM             Reviewed;         449 AA.
AC   Q3BKD5; Q6NE79; V6F2D7;
DT   18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Probable magnetosome protein Mms48 {ECO:0000303|PubMed:24816605};
DE   Flags: Precursor;
GN   Name=mms48 {ECO:0000303|PubMed:24816605}; OrderedLocusNames=MGMSRv2__2399;
GN   ORFNames=mgI457, MGR_4070, ORF1 {ECO:0000303|PubMed:13129949};
OS   Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271
OS   / MSR-1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Magnetospirillum.
OX   NCBI_TaxID=431944;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=16237001; DOI=10.1128/jb.187.21.7176-7184.2005;
RA   Ullrich S., Kube M., Schuebbe S., Reinhardt R., Schueler D.;
RT   "A hypervariable 130-kilobase genomic region of Magnetospirillum
RT   gryphiswaldense comprises a magnetosome island which undergoes frequent
RT   rearrangements during stationary growth.";
RL   J. Bacteriol. 187:7176-7184(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=17449609; DOI=10.1128/jb.00119-07;
RA   Richter M., Kube M., Bazylinski D.A., Lombardot T., Gloeckner F.O.,
RA   Reinhardt R., Schueler D.;
RT   "Comparative genome analysis of four magnetotactic bacteria reveals a
RT   complex set of group-specific genes implicated in magnetosome
RT   biomineralization and function.";
RL   J. Bacteriol. 189:4899-4910(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24625872; DOI=10.1128/genomea.00171-14;
RA   Wang X., Wang Q., Zhang W., Wang Y., Li L., Wen T., Zhang T., Zhang Y.,
RA   Xu J., Hu J., Li S., Liu L., Liu J., Jiang W., Tian J., Li Y., Schuler D.,
RA   Wang L., Li J.;
RT   "Complete genome sequence of Magnetospirillum gryphiswaldense MSR-1.";
RL   Genome Announc. 2:0-0(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=13129949; DOI=10.1128/jb.185.19.5779-5790.2003;
RA   Schuebbe S., Kube M., Scheffel A., Wawer C., Heyen U., Meyerdierks A.,
RA   Madkour M.H., Mayer F., Reinhardt R., Schueler D.;
RT   "Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum
RT   gryphiswaldense reveals a large deletion comprising a putative magnetosome
RT   island.";
RL   J. Bacteriol. 185:5779-5790(2003).
RN   [5]
RP   PROBABLE OPERON, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=22043287; DOI=10.1371/journal.pone.0025561;
RA   Lohsse A., Ullrich S., Katzmann E., Borg S., Wanner G., Richter M.,
RA   Voigt B., Schweder T., Schueler D.;
RT   "Functional analysis of the magnetosome island in Magnetospirillum
RT   gryphiswaldense: the mamAB operon is sufficient for magnetite
RT   biomineralization.";
RL   PLoS ONE 6:E25561-E25561(2011).
RN   [6]
RP   FUNCTION, PROBABLE OPERON, DOMAIN, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1;
RX   PubMed=24816605; DOI=10.1128/jb.01716-14;
RA   Lohsse A., Borg S., Raschdorf O., Kolinko I., Tompa E., Posfai M.,
RA   Faivre D., Baumgartner J., Schueler D.;
RT   "Genetic dissection of the mamAB and mms6 operons reveals a gene set
RT   essential for magnetosome biogenesis in Magnetospirillum gryphiswaldense.";
RL   J. Bacteriol. 196:2658-2669(2014).
CC   -!- FUNCTION: Overexpression in wild-type cells increases the number of
CC       cells with double magnetosome chains significantly. The 4 genes of this
CC       operon collectively influence magnetosome size and number.
CC       {ECO:0000269|PubMed:24816605}.
CC   -!- SUBCELLULAR LOCATION: Magnetosome membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Part of the probable mms6 operon.
CC       {ECO:0000305|PubMed:22043287, ECO:0000305|PubMed:24816605}.
CC   -!- DISRUPTION PHENOTYPE: Normal magnetic response, fewer, larger
CC       magnetosomes; cells accumulate 20% more iron (PubMed:24816605).
CC       Deletion of the 4 gene operon (mms6, mmsF, mms36 and mms48) gives an
CC       intermediate magnetic response with fewer, smaller magnetosomes in
CC       irregular pseudo-chains (PubMed:22043287, PubMed:24816605).
CC       {ECO:0000269|PubMed:22043287, ECO:0000269|PubMed:24816605}.
CC   -!- MISCELLANEOUS: This bacteria makes up to 60 cubo-octahedral
CC       magnetosomes of about 45 nm in diameter which contain membrane-bound
CC       crystals of magnetite (Fe(3)O(4)). {ECO:0000305|PubMed:13129949}.
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DR   EMBL; AM085146; CAJ30093.1; -; Genomic_DNA.
DR   EMBL; CU459003; CAM78002.1; -; Genomic_DNA.
DR   EMBL; HG794546; CDK99614.1; -; Genomic_DNA.
DR   EMBL; BX571797; CAE12014.1; -; Genomic_DNA.
DR   RefSeq; WP_024080608.1; NZ_CP027526.1.
DR   AlphaFoldDB; Q3BKD5; -.
DR   SMR; Q3BKD5; -.
DR   STRING; 1430440.MGMSRv2_2399; -.
DR   EnsemblBacteria; CDK99614; CDK99614; MGMSRv2__2399.
DR   KEGG; mgry:MSR1_03150; -.
DR   KEGG; mgy:MGMSRv2__2399; -.
DR   eggNOG; COG3898; Bacteria.
DR   HOGENOM; CLU_028454_0_0_5; -.
DR   OrthoDB; 696976at2; -.
DR   Proteomes; UP000018922; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0110146; C:magnetosome membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR010817; HemY_N.
DR   InterPro; IPR016982; Mms48.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   Pfam; PF07219; HemY_N; 1.
DR   PIRSF; PIRSF031802; UCP031802; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   3: Inferred from homology;
KW   Biomineralization; Magnetosome; Membrane; Reference proteome; Signal;
KW   TPR repeat; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..449
FT                   /note="Probable magnetosome protein Mms48"
FT                   /id="PRO_0000447735"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          323..356
FT                   /note="TPR"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00339,
FT                   ECO:0000305|PubMed:24816605"
SQ   SEQUENCE   449 AA;  48032 MW;  832CFC76B1B64A9B CRC64;
     MLLRLIVLLI FMSPVVFATL WFSDNVGSVQ VEWLGWHVDS NMPVLLAVIL VVFLIFSALS
     RLSALVADLP SKLGKSRQAR GLEKGMSALL AALDAAESGD VGEGRRFAAE AARLLNNPGL
     AARLDRLLPR PPAQPVAPTR LEAAKGRLFA RKPGPPPPPT PVVDKIQPVV VEKLAAAPAG
     PSREDLEAFS AKIRAGEWGA AQAWIGEAVL AGRLTPLVAA RWRSVALEGQ ALEASPGDPA
     RPLRLAREAM AADQTFLPPA LHVIRAEVSE GRKAEAETLL ASVWPHVPAR VLLDACAPLW
     RDEDQDACLK RLEALAAIAP HHPDGHLAAG EAAFAVQKWG VARRHIMAAL KIAPDALGCR
     LMAEIEEREP GGSARSAEIW RRREHEASLS PAWVCGACAR VVEAWAACCP SCAGVATIEW
     TRSVKAEEAL LPPATTASSM ETPRLFRST