ID   MMSB_MYCBO              Reviewed;         294 AA.
AC   P63936; A0A1R3XYF6; O53814; X2BFW4;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Probable 3-hydroxyisobutyrate dehydrogenase;
DE            Short=HIBADH;
DE            EC=1.1.1.31;
GN   Name=mmsB; OrderedLocusNames=BQ2027_MB0773C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC         oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC         ChEBI:CHEBI:57945; EC=1.1.1.31;
CC   -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC   -!- SIMILARITY: Belongs to the HIBADH-related family. {ECO:0000305}.
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DR   EMBL; LT708304; SIT99372.1; -; Genomic_DNA.
DR   RefSeq; NP_854431.1; NC_002945.3.
DR   RefSeq; WP_003403844.1; NC_002945.4.
DR   AlphaFoldDB; P63936; -.
DR   SMR; P63936; -.
DR   EnsemblBacteria; SIT99372; SIT99372; BQ2027_MB0773C.
DR   GeneID; 45424715; -.
DR   PATRIC; fig|233413.5.peg.843; -.
DR   OMA; WSSEVNN; -.
DR   UniPathway; UPA00362; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006574; P:valine catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR006115; 6PGDH_NADP-bd.
DR   InterPro; IPR011548; HIBADH.
DR   InterPro; IPR015815; HIBADH-related.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR029154; NADP-bd.
DR   Pfam; PF14833; NAD_binding_11; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PIRSF; PIRSF000103; HIBADH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01692; HIBADH; 1.
DR   PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE   3: Inferred from homology;
KW   Branched-chain amino acid catabolism; NAD; Oxidoreductase.
FT   CHAIN           1..294
FT                   /note="Probable 3-hydroxyisobutyrate dehydrogenase"
FT                   /id="PRO_0000173057"
FT   ACT_SITE        168
FT                   /evidence="ECO:0000250"
FT   BINDING         3..31
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         93
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         243
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   294 AA;  29679 MW;  68DEF129A8BA3E68 CRC64;
     MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV
     VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG
     GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI
     AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL
     MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADA