MMSB_MYCTU
ID MMSB_MYCTU Reviewed; 294 AA.
AC P9WNY5; L0T7L8; O53814; P63935;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=Probable 3-hydroxyisobutyrate dehydrogenase;
DE Short=HIBADH;
DE EC=1.1.1.31;
GN Name=mmsB; OrderedLocusNames=Rv0751c; ORFNames=MTV041.25c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-
CC oxopropanoate + H(+) + NADH; Xref=Rhea:RHEA:17681, ChEBI:CHEBI:11805,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57700,
CC ChEBI:CHEBI:57945; EC=1.1.1.31;
CC -!- PATHWAY: Amino-acid degradation; L-valine degradation.
CC -!- SIMILARITY: Belongs to the HIBADH-related family. {ECO:0000305}.
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DR EMBL; AL123456; CCP43497.1; -; Genomic_DNA.
DR PIR; B70825; B70825.
DR RefSeq; NP_215265.1; NC_000962.3.
DR RefSeq; WP_003403844.1; NZ_NVQJ01000035.1.
DR PDB; 5Y8G; X-ray; 2.01 A; A/B=1-294.
DR PDB; 5Y8H; X-ray; 2.10 A; A/B=1-294.
DR PDB; 5Y8I; X-ray; 2.04 A; A/B=1-294.
DR PDB; 5Y8J; X-ray; 1.86 A; A/B=1-294.
DR PDB; 5Y8K; X-ray; 2.04 A; A/B=1-294.
DR PDB; 5Y8L; X-ray; 1.85 A; A/B=1-294.
DR PDB; 5Y8M; X-ray; 2.04 A; A/B=1-294.
DR PDB; 5Y8N; X-ray; 2.68 A; A/B=1-294.
DR PDB; 5Y8O; X-ray; 2.05 A; A/B=1-294.
DR PDB; 5Y8P; X-ray; 2.15 A; A/B=1-294.
DR PDBsum; 5Y8G; -.
DR PDBsum; 5Y8H; -.
DR PDBsum; 5Y8I; -.
DR PDBsum; 5Y8J; -.
DR PDBsum; 5Y8K; -.
DR PDBsum; 5Y8L; -.
DR PDBsum; 5Y8M; -.
DR PDBsum; 5Y8N; -.
DR PDBsum; 5Y8O; -.
DR PDBsum; 5Y8P; -.
DR AlphaFoldDB; P9WNY5; -.
DR SMR; P9WNY5; -.
DR STRING; 83332.Rv0751c; -.
DR PaxDb; P9WNY5; -.
DR GeneID; 45424715; -.
DR GeneID; 888658; -.
DR KEGG; mtu:Rv0751c; -.
DR TubercuList; Rv0751c; -.
DR eggNOG; COG2084; Bacteria.
DR OMA; WSSEVNN; -.
DR PhylomeDB; P9WNY5; -.
DR BRENDA; 1.1.1.31; 3445.
DR UniPathway; UPA00362; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0008442; F:3-hydroxyisobutyrate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0006574; P:valine catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.1040.10; -; 1.
DR InterPro; IPR002204; 3-OH-isobutyrate_DH-rel_CS.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR013328; 6PGD_dom2.
DR InterPro; IPR006115; 6PGDH_NADP-bd.
DR InterPro; IPR011548; HIBADH.
DR InterPro; IPR015815; HIBADH-related.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR029154; NADP-bd.
DR Pfam; PF14833; NAD_binding_11; 1.
DR Pfam; PF03446; NAD_binding_2; 1.
DR PIRSF; PIRSF000103; HIBADH; 1.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01692; HIBADH; 1.
DR PROSITE; PS00895; 3_HYDROXYISOBUT_DH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Branched-chain amino acid catabolism; NAD; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..294
FT /note="Probable 3-hydroxyisobutyrate dehydrogenase"
FT /id="PRO_0000173056"
FT ACT_SITE 168
FT /evidence="ECO:0000250"
FT BINDING 3..31
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 93
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 243
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT STRAND 3..7
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 13..22
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 34..41
FT /evidence="ECO:0007829|PDB:5Y8L"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 51..55
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 59..63
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 68..78
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 79..81
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 87..90
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 96..108
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 112..115
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 121..126
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 138..148
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 152..160
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 163..191
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 196..204
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 211..214
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 218..223
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 227..229
FT /evidence="ECO:0007829|PDB:5Y8L"
FT TURN 230..232
FT /evidence="ECO:0007829|PDB:5Y8L"
FT STRAND 235..237
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 238..255
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 260..276
FT /evidence="ECO:0007829|PDB:5Y8L"
FT HELIX 281..290
FT /evidence="ECO:0007829|PDB:5Y8L"
SQ SEQUENCE 294 AA; 29679 MW; 68DEF129A8BA3E68 CRC64;
MTTIAFLGLG NMGAPMSANL VGAGHVVRGF DPAPTAASGA AAHGVAVFRS APEAVAEADV
VITMLPTGEV VRRCYTDVLA AARPATLFID SSTISVTDAR EVHALAESHG MLQLDAPVSG
GVKGAAAATL AFMVGGDEST LRRARPVLEP MAGKIIHCGA AGAGQAAKVC NNMVLAVQQI
AIAEAFVLAE KLGLSAQSLF DVITGATGNC WAVHTNCPVP GPVPTSPANN DFKPGFSTAL
MNKDLGLAMD AVAATGATAP LGSHAADIYA KFAADHADLD FSAVIHTLRA RADA
