MNAR1_DANRE
ID MNAR1_DANRE Reviewed; 897 AA.
AC A0A140LFM6;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2018, sequence version 3.
DT 03-AUG-2022, entry version 30.
DE RecName: Full=Major intrinsically disordered Notch2-binding receptor 1;
DE AltName: Full=Membrane integral NOTCH2-associated receptor 1;
DE AltName: Full=Ubiquitination and mTOR signaling protein {ECO:0000303|PubMed:30080879};
DE Short=ubtor {ECO:0000303|PubMed:30080879};
GN Name=minar1; Synonyms=ubtora {ECO:0000303|PubMed:30080879};
GN ORFNames=si:cabz01029535.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=30080879; DOI=10.1371/journal.pgen.1007583;
RA Zhang H., Zhang Q., Gao G., Wang X., Wang T., Kong Z., Wang G., Zhang C.,
RA Wang Y., Peng G.;
RT "UBTOR/KIAA1024 regulates neurite outgrowth and neoplasia through mTOR
RT signaling.";
RL PLoS Genet. 14:E1007583-E1007583(2018).
CC -!- FUNCTION: Intrinsically disordered protein which may negatively
CC regulate mTOR signaling pathway by stabilizing the mTOR complex
CC component DEPTOR. Negatively regulates angiogenesis. Negatively
CC regulates cell growth (By similarity). May play a role in neuronal
CC development (PubMed:30080879). {ECO:0000250|UniProtKB:Q9UPX6,
CC ECO:0000269|PubMed:30080879}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9UPX6};
CC Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q9UPX6}.
CC -!- DEVELOPMENTAL STAGE: At 5 dpf, expressed in larvae brain and spinal
CC cord. {ECO:0000269|PubMed:30080879}.
CC -!- DISRUPTION PHENOTYPE: Mutant animals show no gross developmental
CC abnormalities. They have enhanced fear-evoked freezing and compromised
CC C-start responses. Mutant brains exhibit up-regulation of mTOR
CC signaling. {ECO:0000269|PubMed:30080879}.
CC -!- SIMILARITY: Belongs to the MINAR family. {ECO:0000305}.
CC -!- CAUTION: MINAR1 topology is a matter of debate, some authors think the
CC N-terminus is extracellular, while preliminary experimental results
CC suggest a cytosolic location. {ECO:0000250|UniProtKB:Q9UPX6}.
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DR EMBL; CU184872; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_002667438.1; XM_002667392.4.
DR AlphaFoldDB; A0A140LFM6; -.
DR SMR; A0A140LFM6; -.
DR PaxDb; A0A140LFM6; -.
DR GeneID; 100331504; -.
DR KEGG; dre:100331504; -.
DR CTD; 23251; -.
DR ZFIN; ZDB-GENE-160114-82; minar1.
DR eggNOG; ENOG502QSCS; Eukaryota.
DR OMA; IPDKRRV; -.
DR OrthoDB; 1321736at2759; -.
DR PhylomeDB; A0A140LFM6; -.
DR PRO; PR:A0A140LFM6; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0030308; P:negative regulation of cell growth; ISS:UniProtKB.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IBA:GO_Central.
DR GO; GO:0010977; P:negative regulation of neuron projection development; ISS:UniProtKB.
DR GO; GO:0031397; P:negative regulation of protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0032007; P:negative regulation of TOR signaling; ISS:UniProtKB.
DR InterPro; IPR039706; MINAR1-like.
DR InterPro; IPR009626; MINAR1-like_C.
DR PANTHER; PTHR31530; PTHR31530; 1.
DR Pfam; PF06789; MINAR1_C; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..897
FT /note="Major intrinsically disordered Notch2-binding
FT receptor 1"
FT /id="PRO_0000445580"
FT TOPO_DOM 1..872
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 873..893
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 894..897
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT REGION 405..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 450..502
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 688..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 413..428
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..502
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 688..723
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 724..765
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 897 AA; 101179 MW; 912D0863A5B5F33F CRC64;
MDAMPEYSLF LVRILEELDT KYSTVSYQDL CKSLCARFDL VHLAKLRSLL FYTACLDPAF
PATLFKDKMR CSVEDQQSKK LMVAADIVTM FNLIQMNGGM AKDKLPMGPR PKFHKNQSFE
SCRSDTDVYK YNDNDRAYKL LDTRGHHVSR NSPKSDCNSC QQFIPTSDPN FLLGVTKDLK
CRAASLDKLQ HLPQYASVSP PPCEMQSTYF PMDIDSESTT DQESLHANPG IKDVYVSSGE
PFTVHSCVQK RNIFKEDFHN LVAFSPHVIT TECRANPKSG GNYHHRRELS KPPTFFNHSF
ELPYSNPYFE PVNSPLQNKG RVKHESLDDL QASTYFGPTT VSECVSNRRT SSKHGRQPAW
PVKSLSLNTE EGPDFERSFL NGKVPKDNHR HNIGTMENDQ HFQCAKDKPT ASPSGFSKKS
NGSKTKDMSS VACGPGIEKR EVGRRYKDKS INCPSFQSSN VDNGMSVGTQ TEQHESRKVK
DYPSQNKFKE RPPFKHSEED SEIVSDNISD IFRFLDDMSV CESLGVVQPS CYNSNGSLSQ
VTKSDGDSSP ERNTIKIGKT GIKLDRLFQS LENSDDELKE SVCKLVLRIG EIEKKLESLS
GVRGEISQVL SKLTRLDEKI QEPEVNGKSS DGGLVEQIKT DANLSPHVFQ CHTTGHNMKV
EKSPEWCCSE ADGSESLRVK ALKKSVFTRR SSRSLNEENS ATESKVASIT NSPRDWRTVS
YSSHNGEEGK ERDRHSEGKE RHRKSREAER QYEAHQGHRS SKPPKDSYLV EQVFSPHHFP
PTVKTHVKGS PLYTDLRLTG HADGKRSQPS WTIEEYKRNS GDKNKLSALD LQVQESLNPN
NLEYWMEDIY TPGYDSLLKR KEAEFRRAKA CKIGALIFAA ACTVILVIVV PICTMKS
