ID   MNB1A_MAIZE             Reviewed;         238 AA.
AC   P38564;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Dof zinc finger protein MNB1A;
GN   Name=MNB1A;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. H84; TISSUE=Green leaf;
RX   PubMed=8340424; DOI=10.1016/s0021-9258(18)82353-5;
RA   Yanagisawa S., Izui K.;
RT   "Molecular cloning of two DNA-binding proteins of maize that are
RT   structurally different but interact with the same sequence motif.";
RL   J. Biol. Chem. 268:16028-16036(1993).
RN   [2]
RP   SEQUENCE REVISION, AND MUTAGENESIS OF CYS-49; CYS-52 AND CYS-60.
RC   STRAIN=cv. H84; TISSUE=Green leaf;
RX   PubMed=7567449; DOI=10.1093/nar/23.17.3403;
RA   Yanagisawa S.;
RT   "A novel DNA-binding domain that may form a single zinc finger motif.";
RL   Nucleic Acids Res. 23:3403-3410(1995).
CC   -!- FUNCTION: Transcription factor that binds specifically to a 5'-AA[AG]G-
CC       3' consensus core sequence at the MNF1-binding site.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in all tissues examined.
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DR   EMBL; X66076; CAA46875.1; -; mRNA.
DR   PIR; S66358; S66358.
DR   RefSeq; NP_001105709.1; NM_001112239.1.
DR   AlphaFoldDB; P38564; -.
DR   PRIDE; P38564; -.
DR   MaizeGDB; 60791; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; P38564; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IDA:AgBase.
DR   GO; GO:0003677; F:DNA binding; IMP:AgBase.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:AgBase.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:AgBase.
DR   GO; GO:0008270; F:zinc ion binding; IMP:AgBase.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:AgBase.
DR   InterPro; IPR045174; Dof.
DR   InterPro; IPR003851; Znf_Dof.
DR   PANTHER; PTHR31992; PTHR31992; 1.
DR   Pfam; PF02701; zf-Dof; 1.
DR   PROSITE; PS01361; ZF_DOF_1; 1.
DR   PROSITE; PS50884; ZF_DOF_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..238
FT                   /note="Dof zinc finger protein MNB1A"
FT                   /id="PRO_0000074299"
FT   ZN_FING         47..101
FT                   /note="Dof-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT   REGION          1..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          85..155
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        96..112
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         49
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT   BINDING         52
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT   BINDING         74
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT   BINDING         77
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00071"
FT   MUTAGEN         49
FT                   /note="C->S: Abolishes DNA binding."
FT                   /evidence="ECO:0000269|PubMed:7567449"
FT   MUTAGEN         52
FT                   /note="C->A: Abolishes DNA binding."
FT                   /evidence="ECO:0000269|PubMed:7567449"
FT   MUTAGEN         60
FT                   /note="C->S: No effect on DNA binding."
FT                   /evidence="ECO:0000269|PubMed:7567449"
SQ   SEQUENCE   238 AA;  24797 MW;  9E8733228DB573C3 CRC64;
     MQEASSAAAA GAEPGRRAAQ HQFAGVDLRR PKGYAAPAPA PAVGEGDPCP RCASRDTKFC
     YYNNYNTSQP RHFCKGCRRY WTKGGTLRNV PVGGGTRKKP SSSSSSSSYV AAADADRQPK
     KKPASKKRRV VAPAPELATA ADPGKTATTT TTTSEITTET GALEDSDSLA HLLLQPGTED
     AEAVALGLGL SDFPSAGKAV LDDEDSFVWP AASFDMGACW AGAGFADPDP ACIFLNLP