MNEP_BACSU
ID MNEP_BACSU Reviewed; 297 AA.
AC C0SP78; P96691; Q797G0;
DT 07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Manganese efflux system protein MneP {ECO:0000303|PubMed:27748968};
GN Name=mneP {ECO:0000303|PubMed:27748968}; Synonyms=ydfM;
GN OrderedLocusNames=BSU05470;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 209.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27748968; DOI=10.1111/mmi.13554;
RA Huang X., Shin J.H., Pinochet-Barros A., Su T.T., Helmann J.D.;
RT "Bacillus subtilis MntR coordinates the transcriptional regulation of
RT manganese uptake and efflux systems.";
RL Mol. Microbiol. 103:253-268(2017).
CC -!- FUNCTION: Primary efflux pump for manganese. May prevent manganese
CC intoxication. {ECO:0000269|PubMed:27748968}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Transcriptionally activated by MntR in response to manganese
CC excess. Expressed at a basal level in the absence of MntR.
CC {ECO:0000269|PubMed:27748968}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant is manganese sensitive and
CC accumulates high levels of intracellular manganese. Effects are
CC exacerbated in a mneP-mneS double mutant.
CC {ECO:0000269|PubMed:27748968}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
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DR EMBL; AB001488; BAA19381.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12354.2; -; Genomic_DNA.
DR PIR; C69781; C69781.
DR RefSeq; NP_388428.2; NC_000964.3.
DR RefSeq; WP_003244477.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; C0SP78; -.
DR SMR; C0SP78; -.
DR STRING; 224308.BSU05470; -.
DR PaxDb; C0SP78; -.
DR PRIDE; C0SP78; -.
DR EnsemblBacteria; CAB12354; CAB12354; BSU_05470.
DR GeneID; 939900; -.
DR KEGG; bsu:BSU05470; -.
DR PATRIC; fig|224308.179.peg.587; -.
DR eggNOG; COG0053; Bacteria.
DR InParanoid; C0SP78; -.
DR OMA; YGHQRIE; -.
DR PhylomeDB; C0SP78; -.
DR BioCyc; BSUB:BSU05470-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR Gene3D; 3.30.70.1350; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027470; Cation_efflux_CTD.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR Pfam; PF16916; ZT_dimer; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..297
FT /note="Manganese efflux system protein MneP"
FT /id="PRO_0000378462"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 209
FT /note="D -> G (in Ref. 1; BAA19381)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 32854 MW; 901A9FDA4B889E40 CRC64;
MASEREQISR KVALIALIAN LILMAGKVFF GLVGDSEAVF ADGIHSAADV VASIAVLAVI
GISNKPPDQD HPFGHGKAEV ISEAIVGIIL VIVSVYILIE AILSFVKGPS VPQYSALFAA
LISYVAKEIL YRYSIKQGKK WNSKAIIAIA YDHKGDIVAS LAAFIGVLLA IIGNSRGWSY
LLYADAIASA IVAYLIFKIS MELIRPSVDV LMEKSVDPEL IEEYKAVIFQ CDQVKRIDRI
RAREHGHYKL LDVRLSLDHD LTIKQGHDIA REIRNEIKRQ FSDVEEVLIH VNPYFEE
