MNES_BACSU
ID MNES_BACSU Reviewed; 290 AA.
AC P46348; O05001;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Manganese efflux system protein MneS {ECO:0000303|PubMed:27748968};
GN Name=mneS {ECO:0000303|PubMed:27748968}; Synonyms=ydxT, yeaB;
GN OrderedLocusNames=BSU06320;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969499; DOI=10.1099/13500872-142-11-3027;
RA Borriss R., Porwollik S., Schroeter R.;
RT "The 52 degrees-55 degrees segment of the Bacillus subtilis chromosome: a
RT region devoted to purine uptake and metabolism, and containing the genes
RT cotA, gabP and guaA and the pur gene cluster within a 34960 bp nucleotide
RT sequence.";
RL Microbiology 142:3027-3031(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-276.
RC STRAIN=168;
RX PubMed=8951816; DOI=10.1046/j.1365-2958.1996.d01-1720.x;
RA Ferson A.E., Wray L.V. Jr., Fisher S.H.;
RT "Expression of the Bacillus subtilis gabP gene is regulated independently
RT in response to nitrogen and amino acid availability.";
RL Mol. Microbiol. 22:693-701(1996).
RN [4]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27748968; DOI=10.1111/mmi.13554;
RA Huang X., Shin J.H., Pinochet-Barros A., Su T.T., Helmann J.D.;
RT "Bacillus subtilis MntR coordinates the transcriptional regulation of
RT manganese uptake and efflux systems.";
RL Mol. Microbiol. 103:253-268(2017).
CC -!- FUNCTION: Secondary manganese efflux system. May prevent manganese
CC intoxication. {ECO:0000269|PubMed:27748968}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Transcriptionally activated by MntR in response to manganese
CC excess. Expressed at a basal level in the absence of MntR.
CC {ECO:0000269|PubMed:27748968}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene has no significant effet.
CC However, a mneP-mneS double mutant is manganese sensitive and
CC accumulates high levels of intracellular manganese.
CC {ECO:0000269|PubMed:27748968}.
CC -!- SIMILARITY: Belongs to the cation diffusion facilitator (CDF)
CC transporter (TC 2.A.4) family. {ECO:0000305}.
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DR EMBL; U51115; AAB62307.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12451.1; -; Genomic_DNA.
DR EMBL; U31756; AAC44640.1; -; Genomic_DNA.
DR PIR; B69791; B69791.
DR RefSeq; NP_388513.1; NC_000964.3.
DR RefSeq; WP_003233995.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; P46348; -.
DR SMR; P46348; -.
DR STRING; 224308.BSU06320; -.
DR PaxDb; P46348; -.
DR PRIDE; P46348; -.
DR EnsemblBacteria; CAB12451; CAB12451; BSU_06320.
DR GeneID; 936032; -.
DR KEGG; bsu:BSU06320; -.
DR PATRIC; fig|224308.179.peg.686; -.
DR eggNOG; COG0053; Bacteria.
DR InParanoid; P46348; -.
DR OMA; IEMHLIV; -.
DR PhylomeDB; P46348; -.
DR BioCyc; BSUB:BSU06320-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IBA:GO_Central.
DR Gene3D; 1.20.1510.10; -; 1.
DR Gene3D; 3.30.70.1350; -; 1.
DR InterPro; IPR002524; Cation_efflux.
DR InterPro; IPR027470; Cation_efflux_CTD.
DR InterPro; IPR036837; Cation_efflux_CTD_sf.
DR InterPro; IPR027469; Cation_efflux_TMD_sf.
DR Pfam; PF01545; Cation_efflux; 1.
DR Pfam; PF16916; ZT_dimer; 1.
DR SUPFAM; SSF160240; SSF160240; 1.
DR SUPFAM; SSF161111; SSF161111; 1.
DR TIGRFAMs; TIGR01297; CDF; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..290
FT /note="Manganese efflux system protein MneS"
FT /id="PRO_0000206135"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 290 AA; 31785 MW; 642E20CA9088B99D CRC64;
MERYDELKKG ESGALVSIAA YLVLSAIKLI IGYLFHSEAL TADGLNNTTD IIASVAVLIG
LRISQKPPDE DHPYGHFRAE TIASLIASFI MMVVGLQVLF SAGESIFSAK QETPDMIAAW
TAAGGAVLML IVYRYNKRLA KKVKSQALLA AAADNKSDAF VSIGTFIGIV AAQFHLAWID
TVTAFVIGLL ICKTAWDIFK ESSHSLTDGF DIKDISAYKQ TIEKISGVSR LKDIKARYLG
STVHVDVVVE VSADLNITES HDIANEIERR MKEEHAIDYS HVHMEPLEQK
