MNGR_ECOLI
ID MNGR_ECOLI Reviewed; 240 AA.
AC P13669;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 154.
DE RecName: Full=Mannosyl-D-glycerate transport/metabolism system repressor MngR;
DE AltName: Full=Fatty acyl-responsive regulator;
DE AltName: Full=Protein P30;
GN Name=mngR; Synonyms=farR, g30, ybgB; OrderedLocusNames=b0730, JW0719;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2548486; DOI=10.1042/bj2600737;
RA Buck D., Guest J.R.;
RT "Overexpression and site-directed mutagenesis of the succinyl-CoA
RT synthetase of Escherichia coli and nucleotide sequence of a gene (g30) that
RT is adjacent to the suc operon.";
RL Biochem. J. 260:737-747(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
RX PubMed=9063979; DOI=10.1271/bbb.60.309;
RA Utsumi R., Horie T., Katoh A., Kaino Y., Tanabe H., Noda M.;
RT "Isolation and characterization of the heat-responsive genes in Escherichia
RT coli.";
RL Biosci. Biotechnol. Biochem. 60:309-315(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 121-240.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [6]
RP FUNCTION AS A REGULATOR OF THE SUCCINYL-COA-SYNTHETASE OPERON.
RX PubMed=7805834; DOI=10.1016/0014-5793(94)01264-4;
RA Quail M.A., Dempsey C.E., Guest J.R.;
RT "Identification of a fatty acyl responsive regulator (FarR) in Escherichia
RT coli.";
RL FEBS Lett. 356:183-187(1994).
RN [7]
RP FUNCTION AS A REGULATOR OF THE MANNOSYL-GLYCERATE TRANSPORT AND METABOLISM
RP SYSTEM, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=14645248; DOI=10.1074/jbc.m310980200;
RA Sampaio M.-M., Chevance F., Dippel R., Eppler T., Schlegel A., Boos W.,
RA Lu Y.-J., Rock C.O.;
RT "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-
RT D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA)
RT gene and is regulated by the mngR (farR) gene product acting as
RT repressor.";
RL J. Biol. Chem. 279:5537-5548(2004).
CC -!- FUNCTION: Represses mngA and mngB. Regulates its own expression.
CC {ECO:0000269|PubMed:14645248, ECO:0000269|PubMed:7805834}.
CC -!- INDUCTION: Induced by mannosyl-D-glycerate.
CC -!- DISRUPTION PHENOTYPE: In cells lacking this gene the expression of mngA
CC and mngB increases by 9-fold and 23-fold, respectively.
CC {ECO:0000269|PubMed:14645248}.
CC -!- CAUTION: Originally proposed to be involved in the regulation of the
CC acid citric cycle in response to fatty acids. This was based on in
CC vitro experiments showing that MngR was released from its DNA-binding
CC sites by high concentration sof fatty acids and acyl-CoAs. These
CC results could be explained by the fact that these high-concentrations
CC of fatty acids could have denatured the MngR protein. {ECO:0000305}.
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DR EMBL; X15790; CAA33791.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73824.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35396.2; -; Genomic_DNA.
DR EMBL; D64014; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S04645; S04645.
DR RefSeq; NP_415258.1; NC_000913.3.
DR RefSeq; WP_000509902.1; NZ_SSZK01000033.1.
DR AlphaFoldDB; P13669; -.
DR SMR; P13669; -.
DR BioGRID; 4261225; 5.
DR DIP; DIP-9565N; -.
DR IntAct; P13669; 4.
DR STRING; 511145.b0730; -.
DR PaxDb; P13669; -.
DR PRIDE; P13669; -.
DR EnsemblBacteria; AAC73824; AAC73824; b0730.
DR EnsemblBacteria; BAA35396; BAA35396; BAA35396.
DR GeneID; 945371; -.
DR KEGG; ecj:JW0719; -.
DR KEGG; eco:b0730; -.
DR PATRIC; fig|1411691.4.peg.1543; -.
DR EchoBASE; EB1100; -.
DR eggNOG; COG2188; Bacteria.
DR HOGENOM; CLU_063236_5_0_6; -.
DR InParanoid; P13669; -.
DR OMA; FMIETGY; -.
DR PhylomeDB; P13669; -.
DR BioCyc; EcoCyc:PD01103; -.
DR PRO; PR:P13669; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0009408; P:response to heat; IMP:EcoCyc.
DR CDD; cd07377; WHTH_GntR; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.1410.10; -; 1.
DR InterPro; IPR028978; Chorismate_lyase_/UTRA_dom_sf.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR011663; UTRA.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00392; GntR; 1.
DR Pfam; PF07702; UTRA; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00345; HTH_GNTR; 1.
DR SMART; SM00866; UTRA; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF64288; SSF64288; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..240
FT /note="Mannosyl-D-glycerate transport/metabolism system
FT repressor MngR"
FT /id="PRO_0000050641"
FT DOMAIN 4..72
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 32..51
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ SEQUENCE 240 AA; 28273 MW; 69CBB5AFAB2DCDD5 CRC64;
MGHKPLYRQI ADRIREQIAR GELKPGDALP TESALQTEFG VSRVTVRQAL RQLVEQQILE
SIQGSGTYVK EERVNYDIFQ LTSFDEKLSD RHVDTHSEVL IFEVIPADDF LQQQLQITPQ
DRVWHVKRVR YRKQKPMALE ETWMPLALFP DLTWQVMENS KYHFIEEVKK MVIDRSEQEI
IPLMPTEEMS RLLNISQTKP ILEKVSRGYL VDGRVFEYSR NAFNTDDYKF TLIAQRKSSR
