ID   MNHA1_STAAS             Reviewed;         801 AA.
AC   Q6GAX4;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Na(+)/H(+) antiporter subunit A1;
DE   AltName: Full=Mnh complex subunit A1;
GN   Name=mnhA1; OrderedLocusNames=SAS0822;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Mnh complex is a Na(+)/H(+) antiporter involved in Na(+)
CC       excretion.
CC   -!- ACTIVITY REGULATION: Na(+) extrusion is completely inhibited by the
CC       H(+) conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP).
CC   -!- SUBUNIT: May form a heterooligomeric complex that consists of seven
CC       subunits: mnhA1, mnhB1, mnhC1, mnhD1, mnhE1, mnhF1 and mnhG1.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A
CC       family. {ECO:0000305}.
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DR   EMBL; BX571857; CAG42597.1; -; Genomic_DNA.
DR   RefSeq; WP_000054609.1; NC_002953.3.
DR   AlphaFoldDB; Q6GAX4; -.
DR   SMR; Q6GAX4; -.
DR   KEGG; sas:SAS0822; -.
DR   HOGENOM; CLU_007100_2_1_9; -.
DR   OMA; AFILQYM; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005451; F:monovalent cation:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0098662; P:inorganic cation transmembrane transport; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR025383; MbhD-like_TM.
DR   InterPro; IPR005663; MrpA/MnhA1/PhaAB.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   InterPro; IPR001516; Proton_antipo_N.
DR   Pfam; PF13244; DUF4040; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   Pfam; PF00662; Proton_antipo_N; 1.
DR   TIGRFAMs; TIGR00940; 2a6301s01; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..801
FT                   /note="Na(+)/H(+) antiporter subunit A1"
FT                   /id="PRO_0000217070"
FT   TRANSMEM        4..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..127
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        270..289
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        429..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..494
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        526..548
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        589..611
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        621..641
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        646..668
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        672..694
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        707..729
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        767..784
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   801 AA;  89396 MW;  A57CE4814B59367F CRC64;
     MSLLHIAVIL PLIFALIIPI LYRFFKRIHL GWFVLPVPIV IFIYMLTLIK TTMSGNTVMK
     TLNWMPHFGM NFDLYLDGLG LLFSLLISGI GSLVVLYSIG YLSKSEQLGN FYCYLLLFMG
     AMLGVVLSDN VIILYLFWEL TSFSSFLLIS FWRERQASIY GAQKSLIITV FGGLSLLGGI
     ILLAIPTQSF SIQYMIQHAS EIQNSPFFIF AMILIMIGAF TKSAQFPFYI WLPDAMEAPT
     PVSAYLHSAT MVKAGLYLIA RMTPIFAASQ GWVWTVTLVG LITLFWASLN ATKQQDLKGI
     LAFSTVSQLG MIMAMLGIGA ISYHYQGDDS KIYAAAFTAA IFHLINHATF KGALFMITGA
     VDHSTGTRDV KKLGGLLTIM PISFTITVIT ALSMAGVPPF NGFLSKESFL ETTFTASQAN
     LFSVDTLGYL FPIIGIVGSV FTFVYSIKFI MHIFFGQYKP EQLPKKAHEV SILMLLSPAI
     LATLVIVFGL FPGILTNSII EPATSSINHT VIDDVEFHMF HGLTPAFLST LVIYILGILL
     IVTFSYWVKL LQRQPGKLTF NYWYNRSANV IPNYSEKMTN SYVTDYSRNN LVIIFGALIL
     LTFVTIFSVP FNINFKDVSP IRIFEVCIVI LLLSAAFLIL FAKSRLFSII MLSAVGYAVS
     VLFIFFKAPD LALTQFVVES ISTALFLLCF YHLPNLNRYN EKRSFQLTNA LIAGGVGLSV
     IIIGLIAYGN RHFESISKFY QEHVYDLAHG KNMVNVILVD FRGMDTLFES SVLGIAGLAV
     YTMIKLRKKR QTQGNEVKNH E