MNHB1_STAAU
ID MNHB1_STAAU Reviewed; 142 AA.
AC P60678; Q0Q2K6; Q9ZNG5;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Na(+)/H(+) antiporter subunit B1;
DE AltName: Full=Mnh complex subunit B1;
DE AltName: Full=Mrp complex subunit B1;
GN Name=mnhB1; Synonyms=mrpB1;
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION OF ANTIPORTER
RP ACTIVITY.
RC STRAIN=ATCC 21027 / 209-P;
RX PubMed=9852009; DOI=10.1128/jb.180.24.6642-6648.1998;
RA Hiramatsu T., Kodama K., Kuroda T., Mizushima T., Tsuchiya T.;
RT "A putative multisubunit Na+/H+ antiporter from Staphylococcus aureus.";
RL J. Bacteriol. 180:6642-6648(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND PROBABLE
RP ELECTROGENIC ANTIPORTER ACTIVITY.
RC STRAIN=RF4220;
RX PubMed=17293423; DOI=10.1128/jb.00021-07;
RA Swartz T.H., Ito M., Ohira T., Natsui S., Hicks D.B., Krulwich T.A.;
RT "Catalytic properties of Staphylococcus aureus and Bacillus members of the
RT secondary cation/proton antiporter-3 (Mrp) family are revealed by an
RT optimized assay in an Escherichia coli host.";
RL J. Bacteriol. 189:3081-3090(2007).
CC -!- FUNCTION: Mnh complex is a Na(+)Li(+)/H(+) antiporter involved in Na(+)
CC and/or Li(+) excretion. Na(+)/H(+) antiport consumes a transmembrane
CC electrical potential, and is thus inferred to be electrogenic. Does not
CC transport K(+), Ca(2+) or Mg(2+).
CC -!- ACTIVITY REGULATION: Na(+) extrusion is completely inhibited by the
CC H(+) conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP).
CC -!- SUBUNIT: May form a heterooligomeric complex that consists of seven
CC subunits: mnhA1, mnhB1, mnhC1, mnhD1, mnhE1, mnhF1 and mnhG1.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit B
CC family. {ECO:0000305}.
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DR EMBL; AB015981; BAA35096.1; -; Genomic_DNA.
DR EMBL; DQ659238; ABG67111.1; -; Genomic_DNA.
DR PIR; H89861; H89861.
DR RefSeq; WP_001081626.1; NZ_WYDB01000003.1.
DR AlphaFoldDB; P60678; -.
DR SMR; P60678; -.
DR TCDB; 2.A.63.1.3; the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.
DR GeneID; 66839149; -.
DR OMA; HPGFLMP; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR005281; CPA3_sub_B.
DR InterPro; IPR007182; MnhB.
DR Pfam; PF04039; MnhB; 1.
DR TIGRFAMs; TIGR00943; 2a6301s02; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..142
FT /note="Na(+)/H(+) antiporter subunit B1"
FT /id="PRO_0000088861"
FT TRANSMEM 9..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..138
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 142 AA; 15682 MW; 4BF779759F08D24B CRC64;
MNRQQNDLIL QFAAVIIFFM VMVFGFSLFL AGHYTPGGGF VGGLLFASSL VIITIAFDIE
TMRKIFPLDF KILIGIGLVF CIATPIASWF LGKNFFTHVT FDIPLFILEP VHMTTAVFFD
FGVLCAVVGT VMTIIISIGE NE
