ID   ARLY_THENN              Reviewed;         398 AA.
AC   Q9Z4S3; B9K8S6;
DT   23-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Argininosuccinate lyase {ECO:0000255|HAMAP-Rule:MF_00006};
DE            Short=ASAL {ECO:0000255|HAMAP-Rule:MF_00006};
DE            EC=4.3.2.1 {ECO:0000255|HAMAP-Rule:MF_00006};
DE   AltName: Full=Arginosuccinase {ECO:0000255|HAMAP-Rule:MF_00006};
GN   Name=argH {ECO:0000255|HAMAP-Rule:MF_00006}; OrderedLocusNames=CTN_1183;
OS   Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=309803;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E;
RA   Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J.,
RA   Kim J.J., Park K.J., Lee S.Y.;
RT   "The genome sequence of the hyperthermophilic bacterium Thermotoga
RT   neapolitana.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 115-398.
RX   PubMed=10732680; DOI=10.1007/pl00008670;
RA   Dimova D., Weigel P., Takahashi M., Marc F., Van Duyne G.D., Sakanyan V.;
RT   "Thermostability, oligomerization and DNA-binding properties of the
RT   regulatory protein ArgR from the hyperthermophilic bacterium Thermotoga
RT   neapolitana.";
RL   Mol. Gen. Genet. 263:119-130(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(N(omega)-L-arginino)succinate = fumarate + L-arginine;
CC         Xref=Rhea:RHEA:24020, ChEBI:CHEBI:29806, ChEBI:CHEBI:32682,
CC         ChEBI:CHEBI:57472; EC=4.3.2.1; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00006};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine
CC       from L-ornithine and carbamoyl phosphate: step 3/3. {ECO:0000255|HAMAP-
CC       Rule:MF_00006}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00006}.
CC   -!- SIMILARITY: Belongs to the lyase 1 family. Argininosuccinate lyase
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00006}.
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DR   EMBL; CP000916; ACM23359.1; -; Genomic_DNA.
DR   EMBL; AJ009897; CAB38108.1; -; Genomic_DNA.
DR   RefSeq; WP_015919674.1; NC_011978.1.
DR   AlphaFoldDB; Q9Z4S3; -.
DR   SMR; Q9Z4S3; -.
DR   STRING; 309803.CTN_1183; -.
DR   EnsemblBacteria; ACM23359; ACM23359; CTN_1183.
DR   KEGG; tna:CTN_1183; -.
DR   eggNOG; COG0165; Bacteria.
DR   HOGENOM; CLU_027272_2_0_0; -.
DR   OMA; KKNPDVF; -.
DR   UniPathway; UPA00068; UER00114.
DR   Proteomes; UP000000445; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004056; F:argininosuccinate lyase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0042450; P:arginine biosynthetic process via ornithine; IEA:InterPro.
DR   CDD; cd01359; Argininosuccinate_lyase; 1.
DR   Gene3D; 1.10.275.10; -; 1.
DR   HAMAP; MF_00006; Arg_succ_lyase; 1.
DR   InterPro; IPR009049; Argininosuccinate_lyase.
DR   InterPro; IPR024083; Fumarase/histidase_N.
DR   InterPro; IPR020557; Fumarate_lyase_CS.
DR   InterPro; IPR000362; Fumarate_lyase_fam.
DR   InterPro; IPR022761; Fumarate_lyase_N.
DR   InterPro; IPR008948; L-Aspartase-like.
DR   PANTHER; PTHR43814; PTHR43814; 1.
DR   Pfam; PF00206; Lyase_1; 1.
DR   PRINTS; PR00149; FUMRATELYASE.
DR   SUPFAM; SSF48557; SSF48557; 1.
DR   TIGRFAMs; TIGR00838; argH; 1.
DR   PROSITE; PS00163; FUMARATE_LYASES; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Arginine biosynthesis; Cytoplasm; Lyase.
FT   CHAIN           1..398
FT                   /note="Argininosuccinate lyase"
FT                   /id="PRO_0000137842"
FT   CONFLICT        171
FT                   /note="G -> S (in Ref. 2; CAB38108)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   398 AA;  46000 MW;  381B0C7F5F4C0823 CRC64;
     MSEKLWEKGY TVDEEVEKFT VGDDYIVDMR IIKYDIKASI VHSKMLQRTG LLTQEEQKKI
     EEALNELLHL VEEGKFQIKP EDEDCHTAIE NFLVKKLGET GKKIHTARSR NDQVLTALRL
     MYKDELKKIK DLVVELQKSL DGFIERFGQI KFAGFTHTRK AMPTDFATWA GALRDALQDD
     LKLLETVYDI IDQSPLGTGA GYGVPIEVDR EFTAKELGFS RVQWNPIYTQ NSRGKFEYLL
     LHVLSQISYD LNRFASDIIF FSLPEIGFLK LPKELCTGSS IMPHKINPDP LELVRAYHHF
     VVSRMVMAVS LPSNLILGYH RDLQLLKKPV IESIDVVKNI LRIMKIIFDR IEVDREKSED
     SITEEVLATH RVYELVKKGI PFRDAYRMVA EKYGREKD