ID   MNHF2_STAAU             Reviewed;         100 AA.
AC   Q0Q2J5;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Putative antiporter subunit mnhF2;
DE   AltName: Full=Mrp complex subunit F2;
DE   AltName: Full=Putative NADH-ubiquinone oxidoreductase subunit mnhF2;
GN   Name=mnhF2; Synonyms=mrpF2;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION IN E.COLI.
RC   STRAIN=RF4220;
RX   PubMed=17293423; DOI=10.1128/jb.00021-07;
RA   Swartz T.H., Ito M., Ohira T., Natsui S., Hicks D.B., Krulwich T.A.;
RT   "Catalytic properties of Staphylococcus aureus and Bacillus members of the
RT   secondary cation/proton antiporter-3 (Mrp) family are revealed by an
RT   optimized assay in an Escherichia coli host.";
RL   J. Bacteriol. 189:3081-3090(2007).
CC   -!- FUNCTION: Expression of the mnh2 operon in E.coli is not able to
CC       catalyze Na(+)Li(+)/H(+) antiport. It does however confer higher growth
CC       rates than the control strain at up to pH 9.5. The operon may encode an
CC       NADH-ubiquinone oxidoreductase.
CC   -!- SUBUNIT: May form a heterooligomeric complex that consists of seven
CC       subunits: mnhA2, mnhB2, mnhC2, mnhD2, mnhE2, mnhF2 and mnhG2.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F
CC       family. {ECO:0000305}.
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DR   EMBL; DQ659239; ABG67122.1; -; Genomic_DNA.
DR   RefSeq; WP_000616642.1; NZ_WYDB01000005.1.
DR   AlphaFoldDB; Q0Q2J5; -.
DR   SMR; Q0Q2J5; -.
DR   OMA; YERAIYI; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015075; F:ion transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR007208; MrpF/PhaF-like.
DR   PANTHER; PTHR34702; PTHR34702; 1.
DR   Pfam; PF04066; MrpF_PhaF; 1.
DR   PIRSF; PIRSF028784; MrpF; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Ion transport; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..100
FT                   /note="Putative antiporter subunit mnhF2"
FT                   /id="PRO_0000372190"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   100 AA;  10730 MW;  014CDCD636D452CF CRC64;
     MIQTITHIMI ISSLIIFGIA LIICLFRLIK GPTTADRVVT FDTTSAVVMS IVGVLSVLMG
     TVSFLDSIML IAIISFVSSV SISRFIGGGH VFNGNNKRNL