MNM2_CAEEL
ID MNM2_CAEEL Reviewed; 249 AA.
AC Q17895;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Zinc finger protein mnm-2 {ECO:0000305};
GN Name=mnm-2 {ECO:0000312|WormBase:C10A4.8};
GN ORFNames=C10A4.8 {ECO:0000312|WormBase:C10A4.8};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF ASN-141.
RX PubMed=17916347; DOI=10.1016/j.ydbio.2007.08.037;
RA Rauthan M., Moerck C., Pilon M.;
RT "The C. elegans M3 neuron guides the growth cone of its sister cell M2 via
RT the Krueppel-like zinc finger protein MNM-2.";
RL Dev. Biol. 311:185-199(2007).
CC -!- FUNCTION: Required in the M3 pharyngeal motor neuron to guide the
CC growth cone of the sister M2 motor neuron during axon development.
CC {ECO:0000269|PubMed:17916347}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17916347}.
CC -!- TISSUE SPECIFICITY: In larva and adult, expressed in the M3 pharyngeal
CC motor neurons, extrapharyngeal neurons in the head, the PQR tail
CC neurons, rectal cells, vulva cells, the spermetheca-uterine valve, body
CC wall muscle cells and neurons of the ventral nerve cord. In the embryo,
CC expressed in pharyngeal cells, extrapharyngeal head neurons and within
CC the tail. Expressed in body wall muscle cells during late embryonic
CC stages. Expressed in the mother cells of the M2 and M3 pharyngeal motor
CC neurons precursor cells at the embryonic bean stage and subsequently in
CC the M2 and M3 cells as they are born. Expression is sustained only in
CC the two M3 cells up to at least the 5-day-old adult. In contrast,
CC expression gradually declines in the M2 cells beginning from the time
CC of their birth, and is completely undetectable by the time of hatching.
CC {ECO:0000269|PubMed:17916347}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in defective
CC formation of the distal end of the two M2 pharyngeal motor neurons.
CC {ECO:0000269|PubMed:17916347}.
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DR EMBL; BX284606; CCD62398.1; -; Genomic_DNA.
DR PIR; T15486; T15486.
DR RefSeq; NP_509256.2; NM_076855.2.
DR AlphaFoldDB; Q17895; -.
DR SMR; Q17895; -.
DR IntAct; Q17895; 7.
DR STRING; 6239.C10A4.8; -.
DR PaxDb; Q17895; -.
DR EnsemblMetazoa; C10A4.8.1; C10A4.8.1; WBGene00003380.
DR GeneID; 182489; -.
DR KEGG; cel:CELE_C10A4.8; -.
DR UCSC; C10A4.8; c. elegans.
DR CTD; 182489; -.
DR WormBase; C10A4.8; CE35391; WBGene00003380; mnm-2.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_1116607_0_0_1; -.
DR InParanoid; Q17895; -.
DR OMA; MRTHTNL; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q17895; -.
DR SignaLink; Q17895; -.
DR PRO; PR:Q17895; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003380; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IMP:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:1902669; P:positive regulation of axon guidance; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 1: Evidence at protein level;
KW Metal-binding; Neurogenesis; Nucleus; Reference proteome; Repeat; Zinc;
KW Zinc-finger.
FT CHAIN 1..249
FT /note="Zinc finger protein mnm-2"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438328"
FT ZN_FING 168..190
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 196..218
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 224..246
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 20..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..44
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..65
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 141
FT /note="N->SINSDKLQVAMLYIISD: In mnm-2(et2); Defective
FT formation of the distal end of the two M2 motor neurons
FT with M2 growth cones forming at the correct position but
FT stalling or erring ipsilaterally to produce abnormal
FT trajectories and thick growth cone remnants. mnm-2
FT expression is gradually lost in both the M2 and M3 motor
FT neurons in contrast to the wild-type where expression is
FT maintained in the M3 neurons. Functional impairment of the
FT M3 neuron with dramatically reduced pharyngeal pumping."
FT /evidence="ECO:0000269|PubMed:17916347"
SQ SEQUENCE 249 AA; 28491 MW; AAD593B1572AEB46 CRC64;
MSVEASRTCF LIDSLLNKKP KEELETEEED EEEDEEEELS SSEVTSENDM ETESASSSAS
SVGQPKPDFS AFHKIFSNLD FAKLAAVKRN GHHQPMLFRP ECFFPLELSK HLHLVQQTFQ
MNVLQNLGHT LPLPFVPMLK NVAPAQKRLN NKRASYVDHS QKGNLKKYRC DVCDKTFSRS
NTLITHKRIH TGEKPFKCEH CGRAFRQPGN LTRHRLTHTT VKPYVCGLCD KAFNRASNLH
THMRTHTNV
