ARN1_YEAST
ID ARN1_YEAST Reviewed; 627 AA.
AC P38731; D3DKS9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Siderophore iron transporter ARN1;
DE AltName: Full=Ferrichrome permease;
GN Name=ARN1; OrderedLocusNames=YHL040C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA Kaplan J., Philpott C.C.;
RT "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT for two pathways of iron uptake.";
RL J. Biol. Chem. 275:10709-10715(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12721368; DOI=10.1073/pnas.1030323100;
RA Moore R.E., Kim Y., Philpott C.C.;
RT "The mechanism of ferrichrome transport through Arn1p and its metabolism in
RT Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5664-5669(2003).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=10748025; DOI=10.1074/jbc.m001456200;
RA Yun C.-W., Tiedeman J.S., Moore R.E., Philpott C.C.;
RT "Siderophore-iron uptake in Saccharomyces cerevisiae. Identification of
RT ferrichrome and fusarinine transporters.";
RL J. Biol. Chem. 275:16354-16359(2000).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Involved in the transport of siderophore ferrichrome and so
CC has a role in iron homeostasis. {ECO:0000269|PubMed:12721368}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12721368};
CC Multi-pass membrane protein {ECO:0000269|PubMed:16847258}. Endosome
CC membrane {ECO:0000269|PubMed:10748025}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16847258}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; U11583; AAB65052.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06646.1; -; Genomic_DNA.
DR PIR; S48928; S48928.
DR RefSeq; NP_011823.1; NM_001179120.1.
DR AlphaFoldDB; P38731; -.
DR BioGRID; 36383; 35.
DR STRING; 4932.YHL040C; -.
DR TCDB; 2.A.1.16.4; the major facilitator superfamily (mfs).
DR PaxDb; P38731; -.
DR PRIDE; P38731; -.
DR EnsemblFungi; YHL040C_mRNA; YHL040C; YHL040C.
DR GeneID; 856345; -.
DR KEGG; sce:YHL040C; -.
DR SGD; S000001032; ARN1.
DR VEuPathDB; FungiDB:YHL040C; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176305; -.
DR HOGENOM; CLU_012970_2_1_1; -.
DR InParanoid; P38731; -.
DR OMA; VPTWPFI; -.
DR BioCyc; YEAST:G3O-31058-MON; -.
DR PRO; PR:P38731; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38731; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015343; F:siderophore transmembrane transporter activity; IMP:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IBA:GO_Central.
DR GO; GO:0015891; P:siderophore transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR036259; MFS_trans_sf.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Endosome; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..627
FT /note="Siderophore iron transporter ARN1"
FT /id="PRO_0000084862"
FT TOPO_DOM 1..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..135
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..197
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 219..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..290
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..323
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 345..367
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 389..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..424
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..563
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 564..584
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 585..627
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 627 AA; 70899 MW; FD06176163668174 CRC64;
MESVHSRDPV KEEKKHVFMG MEHELNPETH NDSNSDSYGL PQLSEKYNAL RQNRSLIIQQ
TEIIGSAYNK WYLQAILLLS AFICGYGYGL DGNIRYIYTG YATSSYSEHS LLSTINVINA
VVSAASQIIY ARLSDVFGRL YLFISAVILY VVGTIIQSQA YDVQRYAAGA IFYNAGYVGV
ILILLIILSD FSSLKWRLLY QFVPTWPFII NTWIAGNITS RANPVVNWSW DVGMWAFIFP
LSCVPIVLCM LHMQWRARKT PEWHALKGQK SYYQEHGFIK ILKQLFWMLD VVGVLLMGCS
LGCILVPLTL AGGVKTTWND SRLIGPFVLG FVLIPILWIW EYRFARDPIL PYRLVKDRAV
WSSMGISFLI DFIYYMAADY LYTVMIVAVN ESVKSATRIA TLSSFVSTVA SPFFALLVTR
CTRLKPFIMF GCALWMVAMG LLYHFRGGSQ SHSGIIGALC VWGVGTTLFT YPVTVSVQSA
VSHENMATVT ALNYTLYRIG SAVGSAVSGA IWTQTLYKQI LKRMGDVALA TTAYESPYTF
IETYTWGTPQ RNALMNAYKY VQRLETIVAL VFCVPLIAFS LCLRDPKLTD TVAVEYIEDG
EYVDTKDNDP ILDWFEKLPS KFTFKRE
