ARN2_YEAST
ID ARN2_YEAST Reviewed; 620 AA.
AC P38724; D3DKS4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Siderophore iron transporter ARN2;
DE AltName: Full=Triacetylfusarinine C permease;
DE AltName: Full=Triacetylfusarinine C transporter 1;
GN Name=ARN2; Synonyms=TAF1; OrderedLocusNames=YHL047C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 619.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=10816729; DOI=10.1023/a:1009252118050;
RA Heymann P., Ernst J.F., Winkelmann G.;
RT "Identification of a fungal triacetylfusarinine C siderophore transport
RT gene (TAF1) in Saccharomyces cerevisiae as a member of the major
RT facilitator superfamily.";
RL BioMetals 12:301-306(1999).
RN [4]
RP FUNCTION.
RX PubMed=10744769; DOI=10.1074/jbc.275.14.10709;
RA Yun C.-W., Ferea T., Rashford J., Ardon O., Brown P.O., Botstein D.,
RA Kaplan J., Philpott C.C.;
RT "Desferrioxamine-mediated iron uptake in Saccharomyces cerevisiae. Evidence
RT for two pathways of iron uptake.";
RL J. Biol. Chem. 275:10709-10715(2000).
CC -!- FUNCTION: Involved in the transport of siderophore triacestylfusarinine
CC C and so has a role in iron homeostasis. {ECO:0000269|PubMed:10744769,
CC ECO:0000269|PubMed:10816729}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB65059.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U11583; AAB65059.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006934; DAA06641.2; -; Genomic_DNA.
DR PIR; S48921; S48921.
DR RefSeq; NP_011816.2; NM_001179127.2.
DR AlphaFoldDB; P38724; -.
DR BioGRID; 36378; 23.
DR DIP; DIP-7479N; -.
DR STRING; 4932.YHL047C; -.
DR TCDB; 2.A.1.16.3; the major facilitator superfamily (mfs).
DR PaxDb; P38724; -.
DR PRIDE; P38724; -.
DR EnsemblFungi; YHL047C_mRNA; YHL047C; YHL047C.
DR GeneID; 856338; -.
DR KEGG; sce:YHL047C; -.
DR SGD; S000001039; ARN2.
DR VEuPathDB; FungiDB:YHL047C; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176305; -.
DR HOGENOM; CLU_012970_2_1_1; -.
DR InParanoid; P38724; -.
DR OMA; SIGMWAF; -.
DR BioCyc; YEAST:G3O-31063-MON; -.
DR PRO; PR:P38724; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38724; protein.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR GO; GO:0015343; F:siderophore transmembrane transporter activity; IDA:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006879; P:cellular iron ion homeostasis; IMP:SGD.
DR GO; GO:0009237; P:siderophore metabolic process; IMP:SGD.
DR GO; GO:0015891; P:siderophore transport; IDA:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Endosome; Ion transport; Iron; Iron transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..620
FT /note="Siderophore iron transporter ARN2"
FT /id="PRO_0000084863"
FT TRANSMEM 71..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..414
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 491..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..578
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 620 AA; 69566 MW; 19C3DDF83A1FC760 CRC64;
MIEVPEDNRS SQTKRKNTEK NCNELMVDEK MDDDSSPRDE MKDKLKGTKS LIIRKSELMA
KKYDTWQLKA IFLFSAFICT FAYGLDSSIR GTYMTYAMNS YSAHSLISTV SVIVLMISAV
SQVIFGGLSD IFGRLTLFLV SIVLYIVGTI IQSQAYDVQR YAAGAVFYYV GLVGVMLQVV
LMLSDNSSLK WRLFYTLIPS WPSIITTWVS GSVVEAANPL ENWSWNIAMW AFIFPLCCIP
LILCMLHMRW KVRNDVEWKE LQDEKSYYQT HGLVQMLVQL FWKLDVVGVL LFTAGVGCIL
VPLTLAGGVS TNWRNSKIIG PFVLGFVLVP GFIYWESRLA LVPFAPFKLL KDRGVWAPLG
IMFFICFVYQ MAAGYLYTIL VVAVDESASS ATRIINLYSF VTAVVAPFLG LIVTRSSRLK
SYIIFGGSLY FITMGLFYRY RSGQDADGGI IAGMVIWGLS SCLFDYPTIV SIQSVTSHEN
MATVTALNYT VFRIGGAVAA AISGAIWTQS LYPKLLHYMG DADLATAAYG SPLTFILSNP
WGTPVRSAMV EAYRHVQKYE VIVALVFSAP MFLLTFCVRD PRLTEDFAQK LPDREYVQTK
EDDPINDWIA KRFAKALGRS