MNMG1_CALS4
ID MNMG1_CALS4 Reviewed; 633 AA.
AC Q8RAT8;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG 1 {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A 1 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG1 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA1 {ECO:0000255|HAMAP-Rule:MF_00129};
GN OrderedLocusNames=TTE1110;
OS Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM
OS 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis).
OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Caldanaerobacter.
OX NCBI_TaxID=273068;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15242 / JCM 11007 / NBRC 100824 / MB4;
RX PubMed=11997336; DOI=10.1101/gr.219302;
RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., Chen Y.,
RA Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., Tan H., Chen R.,
RA Wang J., Yu J., Yang H.;
RT "A complete sequence of the T. tengcongensis genome.";
RL Genome Res. 12:689-700(2002).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; AE008691; AAM24348.1; -; Genomic_DNA.
DR RefSeq; WP_011025458.1; NC_003869.1.
DR AlphaFoldDB; Q8RAT8; -.
DR SMR; Q8RAT8; -.
DR STRING; 273068.TTE1110; -.
DR EnsemblBacteria; AAM24348; AAM24348; TTE1110.
DR KEGG; tte:TTE1110; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_9; -.
DR OMA; FRPGYAI; -.
DR OrthoDB; 146811at2; -.
DR Proteomes; UP000000555; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..633
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG 1"
FT /id="PRO_0000117200"
FT BINDING 14..19
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 273..287
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 633 AA; 71072 MW; 57895890E08C4477 CRC64;
MRFVAGEYDV CVVGLGHAGS EAALASARLG LSTVGFATNL DAIALMACNP SIGGPAKAQL
VREIDALGGQ MAINTDKSLL QMRTLNTSKG PAVRSLRAQV DKKLYQMNMK HTLERQENLD
IKQAEIVDIL VEDNKVTGVV TKLGAIYKCK ACIITTGTFL KGRVIIGEVD FESGPSGLFP
ASELSEALKR LGFKLMRFKT GTPPRVDKRS IDFSKMEIQP GDEVITPFSF MHDKIEIEQM
PCWLTYTNEK THKIIRDNIH RAPLFTGAIT GVGVRYCPSI EDKVVKFPHR ERHQIFIEPE
GRDTYEMYVQ GMSSSLPEDV QLEFLRTVPG LENVRVMRPA YAIEYDCIDP TQLKATLESK
WIEGLYFAGQ VNGTSGYEEA AAQGLMAGIN AAMKILNKPP VVLDRSQAYI GILIDDLVTK
GTNEPYRMLT SRAEYRLLLR QDNADFRLTE IGKEIGLVTE ERYEKFLRKK IQLEKEMRRL
STVMVRPTEE VNNFLISRGS TPLVSGVDLY TLLKRPEVDY KSTKFLDPDR PDDILDSVAE
QIDINIKYEG YILKQLRQVE QFKAMENKKI PEDIDYYQIS GLSNEAKEKL SKIRPTSVGQ
ASRISGVSPA DISVLLIYLQ QMRKKKSNES RIS
