MNMG1_FUSNN
ID MNMG1_FUSNN Reviewed; 628 AA.
AC Q8RHA1;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG 1 {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A 1 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG1 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA1 {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=FN0007;
OS Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 /
OS BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355).
OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX NCBI_TaxID=190304;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC
RC 2640 / LMG 13131 / VPI 4355;
RX PubMed=11889109; DOI=10.1128/jb.184.7.2005-2018.2002;
RA Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O.,
RA Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N., D'Souza M.,
RA Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C.,
RA Overbeek R.;
RT "Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum
RT strain ATCC 25586.";
RL J. Bacteriol. 184:2005-2018(2002).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; AE009951; AAL94220.1; -; Genomic_DNA.
DR RefSeq; NP_602921.1; NC_003454.1.
DR AlphaFoldDB; Q8RHA1; -.
DR SMR; Q8RHA1; -.
DR STRING; 190304.FN0007; -.
DR EnsemblBacteria; AAL94220; AAL94220; FN0007.
DR KEGG; fnu:FN0007; -.
DR PATRIC; fig|190304.8.peg.599; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_0; -.
DR InParanoid; Q8RHA1; -.
DR OMA; RYQTATP; -.
DR BioCyc; FNUC190304:G1FZS-621-MON; -.
DR Proteomes; UP000002521; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..628
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG 1"
FT /id="PRO_0000117103"
FT BINDING 11..16
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 280..294
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 628 AA; 71076 MW; BCC1A6FA06E1180C CRC64;
MNKDYDVIVV GAGHAGVEAA LASARLGNKT ALITLYLDTI SMMSCNPSIG GPGKSNLVTE
IDVLGGEMGR HIDEFNLQLK DLNTSKGPAA RITRGQADKY KYRRKMREKL EKTENISLIQ
DCVEEILVED IKDTQNSNYI KKITGIKTRL GIIYNAKVIV LATGTFLKGK IVIGDVSYSA
GRQGETSAEK LSDSLRELGI KIERYQTATP PRLDKKTIDF SQLEELKGEE HPRYFSLFTK
KEKNNTVPTW LTYTSDKTIE VIKEMMKFSP IVSGMVNTHG PRHCPSIDRK VLNFPDKEKH
QIFLEMESEN SDEIYVNGLT TAMPAFVQEK ILKTIKGLEN AKIMRYGYAV EYDYAPASQL
YPSLENKKIS GLFFAGQING TSGYEEAAAQ GFIAGVNAAK KIKGEKPVII DRSEAYIGVL
IDDLIHKKTP EPYRVLPSRA EYRLTLRYDN AFMRLFDKIK EVGIVDKDKI EFLEKSINDV
YMEINNLKNI SVSMNEANKF LESLDIEERF VKGVKASEIL KIKDVSYDNL KVFLNLNDYE
DFVKNQIETM IKYEIFIERE NKQIEKFKKL EHMYIPENIN YDEIKGISNI ARAGLDEVRP
LSIGEATRIS GVTSNDITLI IAYMNIKL
