MNMG2_FUSNN
ID MNMG2_FUSNN Reviewed; 633 AA.
AC Q8RI88;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG 2 {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A 2 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG2 {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA2 {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=FN1723;
OS Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 /
OS BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355).
OC Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX NCBI_TaxID=190304;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC
RC 2640 / LMG 13131 / VPI 4355;
RX PubMed=11889109; DOI=10.1128/jb.184.7.2005-2018.2002;
RA Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O.,
RA Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N., D'Souza M.,
RA Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C.,
RA Overbeek R.;
RT "Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum
RT strain ATCC 25586.";
RL J. Bacteriol. 184:2005-2018(2002).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; AE009951; AAL93838.1; -; Genomic_DNA.
DR RefSeq; NP_602539.1; NC_003454.1.
DR AlphaFoldDB; Q8RI88; -.
DR SMR; Q8RI88; -.
DR STRING; 190304.FN1723; -.
DR PRIDE; Q8RI88; -.
DR EnsemblBacteria; AAL93838; AAL93838; FN1723.
DR KEGG; fnu:FN1723; -.
DR PATRIC; fig|190304.8.peg.212; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_0; -.
DR InParanoid; Q8RI88; -.
DR OMA; FRPGYAI; -.
DR BioCyc; FNUC190304:G1FZS-223-MON; -.
DR Proteomes; UP000002521; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..633
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG 2"
FT /id="PRO_0000117104"
FT BINDING 10..15
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 122
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 181
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 277..291
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 374
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 633 AA; 71318 MW; 2EC1E8EFA5BF3B04 CRC64;
MQEFDIIVVG GGHAGCEAAL ASARMGMKTA IFTISLDTIG VMSCNPSLGG PAKSHLAREI
DALGGEMGRN IDKTFIQIRV LNTRKGPAVR SLRAQADKMA YANEMKKTLE HTDNLSVIQG
MVSELVVEEE NGKKIIKGIK IREGLEYKAK AVIIATGTFL RGLIHIGEIN FSAGRMGELS
SEELPLSLEK IGLKLERFKT GTPTRIDGRT IDYSVLEEQP GDKSQVLKFS NRTKDEDALS
RRQISCYIAH TNEKVHEIIR NAKERSPLFN GTIQGLGPRY CPSIEDKIFR YPDKNQHHLF
LEREGYETNE IYLGGLSSSL PVDVQEEMLK NIKGFENAKI MRYAYAIEYD YVPPEEIKYT
LESRTVENLF LAGQINGTSG YEEAGAQGLM AGINAVRKLR NEEPVILDRA DSYIGTLIDD
LVSKGTNEPY RMFTARSEYR LYLREDNADL RLTKLGYELG LVPEEEYQRV EKKRKDVKII
TEILAKTNVG PSNPRVNEIL LKRGENPIKD GSTLLELLRR PEVSFEDIKY ISEEIRGIDL
QGYDHDTTYQ VEITVKYEGY INRALKMIEK HKSMENKKIP VDIDYDDLKT IPKEAKDKLK
RIKPINIGQA SRISGVSPAD IQAILIYLKM RGN
