ID   MNMG_AQUAE              Reviewed;         617 AA.
AC   O66962;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG;
DE   AltName: Full=Glucose-inhibited division protein A;
GN   Name=mnmG; Synonyms=gidA; OrderedLocusNames=aq_761;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEX WITH FAD, AND SUBUNIT.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Crystal structure of gidA from Aquifex aeolicus.";
RL   Submitted (JAN-2008) to the PDB data bank.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000305}.
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DR   EMBL; AE000657; AAC06917.1; -; Genomic_DNA.
DR   PIR; G70366; G70366.
DR   RefSeq; NP_213523.1; NC_000918.1.
DR   RefSeq; WP_010880461.1; NC_000918.1.
DR   PDB; 2ZXH; X-ray; 3.20 A; A/B=1-617.
DR   PDB; 2ZXI; X-ray; 2.30 A; A/B/C/D=1-617.
DR   PDBsum; 2ZXH; -.
DR   PDBsum; 2ZXI; -.
DR   AlphaFoldDB; O66962; -.
DR   SMR; O66962; -.
DR   DIP; DIP-48311N; -.
DR   STRING; 224324.aq_761; -.
DR   EnsemblBacteria; AAC06917; AAC06917; aq_761.
DR   KEGG; aae:aq_761; -.
DR   PATRIC; fig|224324.8.peg.606; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_0; -.
DR   InParanoid; O66962; -.
DR   OMA; FRPGYAI; -.
DR   OrthoDB; 146811at2; -.
DR   EvolutionaryTrace; O66962; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome;
KW   tRNA processing.
FT   CHAIN           1..617
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117046"
FT   BINDING         14..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000269|Ref.2"
FT   BINDING         126
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000269|Ref.2"
FT   BINDING         181
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000269|Ref.2"
FT   BINDING         280..294
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
FT   BINDING         377
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000269|Ref.2"
FT   HELIX           4..6
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          9..13
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           17..28
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          33..38
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           40..42
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          50..53
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           57..67
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           71..78
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          79..86
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           91..93
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          95..100
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           102..114
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          119..124
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          126..141
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          146..148
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          150..154
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          163..166
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          169..172
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           184..190
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          196..202
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          205..207
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           208..210
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          216..219
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          230..234
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          249..253
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           256..264
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           265..268
FT                   /evidence="ECO:0007829|PDB:2ZXH"
FT   TURN            281..283
FT                   /evidence="ECO:0007829|PDB:2ZXH"
FT   HELIX           287..293
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          301..306
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          313..317
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           325..332
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          343..345
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          348..354
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           357..359
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          364..370
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          372..374
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           376..379
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           384..402
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   TURN            412..414
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           416..427
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           435..437
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   TURN            441..445
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           451..461
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          463..465
FT                   /evidence="ECO:0007829|PDB:2ZXH"
FT   HELIX           467..487
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          491..496
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   STRAND          499..504
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           505..508
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   TURN            509..512
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           515..522
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           530..552
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           554..560
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           570..572
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           578..587
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           592..595
FT                   /evidence="ECO:0007829|PDB:2ZXI"
FT   HELIX           603..612
FT                   /evidence="ECO:0007829|PDB:2ZXI"
SQ   SEQUENCE   617 AA;  69644 MW;  1DAB1BDFEFCBA6DD CRC64;
     MAWVVDEFDV VVIGGGHAGI EAALAAARMG AKTAMFVLNA DTIGQMSCNP AIGGIAKGIV
     VREIDALGGE MGKAIDQTGI QFKMLNTRKG KAVQSPRAQA DKKRYREYMK KVCENQENLY
     IKQEEVVDII VKNNQVVGVR TNLGVEYKTK AVVVTTGTFL NGVIYIGDKM IPGGRLGEPR
     SEGLSDFYRR FDFPLIRFKT GTPARLDKRT IDFSALEVAP GDDPPPKFSF WTEPVGSYWF
     PKGKEQVNCW ITYTTPKTHE IIRKNLHRTA LYGGLIKGIG PRYCPSIEDK IVKFPDKERH
     QIFLEPEGLD TIEIYPNGLS TSLPEEVQWE MYRSIPGLEN VVLIRPAYAI EYDVVPPTEL
     YPTLETKKIR GLFHAGNFNG TTGYEEAAGQ GIVAGINAAL RAFGKEPIYL RRDESYIGVM
     IDDLTTKGVT EPYRLFTSRS EYRLYIRQDN AILRLAKLGR ELGLLSEEQY KLVKELEREI
     EKWKEFYKSE RVSVAVGGDT RSYSVATLMT MNYTLDDVKE KFGYEVPQHP YVKEEVEIQL
     KYEPYIERER KLNEKLKKLE DTKIPPDIDY DKIPGLTKEA REKLKKFKPI TVGQASRIDG
     ITPAAITALL VYLGKLD