ID   MNMG_BRADU              Reviewed;         626 AA.
AC   Q89WP5;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=bll0633;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; BA000040; BAC45898.1; -; Genomic_DNA.
DR   RefSeq; NP_767273.1; NC_004463.1.
DR   RefSeq; WP_011083460.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89WP5; -.
DR   SMR; Q89WP5; -.
DR   STRING; 224911.27348882; -.
DR   EnsemblBacteria; BAC45898; BAC45898; BAC45898.
DR   GeneID; 64020495; -.
DR   KEGG; bja:bll0633; -.
DR   PATRIC; fig|224911.44.peg.9173; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_5; -.
DR   InParanoid; Q89WP5; -.
DR   OMA; FRPGYAI; -.
DR   PhylomeDB; Q89WP5; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..626
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117068"
FT   BINDING         14..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         273..287
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   626 AA;  67534 MW;  79EE73DFFF5918C7 CRC64;
     MRPERESFDV IVIGGGHAGC EAAAASARMG AATALVTHRF STVGAMSCNP AIGGLGKGHL
     VREVDALDGL MGRVGDAGGI QFRVLNRRKG PAVRGPRAQA DRKLYAAAMQ AAIRETEGLS
     VIEGEADELI VVEGRVTGLR LADGREFGAG SIVVTTGTFL RGLIHLGEKN WPAGRVGEAP
     AMGLSSSFER AGFTLGRLKT GTPPRLDGTT IDWSAVEMQP GDEPPEPFSV MTDRITTPQI
     QCGITRTTAA THEVIRANVH RSPMYSGQIK SSGPRYCPSI EDKIVRFGDR DGHQIFLEPE
     GLDDSTVYPN GISTSLPEEV QLAILASIPG LERVKMVRPG YAIEYDHIDP RELDPTLQTK
     RLRGLFLAGQ INGTTGYEEA AGQGIVAGLN AALAASGAAL TVFDRADGYL GVMIDDLVTR
     GISEPYRMFT SRAEYRLTLR ADNADQRLTE KGIALGCVRS ARTLHHRTKM EALNAARTLS
     KSLTITPNEA IKHGLSLNRD GQRRSAFELM AYPDIGWSQV RAIWLELSAI DPVIATHLEI
     DAKYDVYLER QSADVEAFRR DEGLVLSEVD YQLVPGLSNE VRAKLEKARP FTVGQAGRID
     GMTPAALGIL AAYLRREARK TSKAIA