ID   MNMG_CHLAA              Reviewed;         651 AA.
AC   A9WKL7;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=Caur_3460;
OS   Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl).
OC   Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae;
OC   Chloroflexaceae; Chloroflexus.
OX   NCBI_TaxID=324602;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29366 / DSM 635 / J-10-fl;
RX   PubMed=21714912; DOI=10.1186/1471-2164-12-334;
RA   Tang K.H., Barry K., Chertkov O., Dalin E., Han C.S., Hauser L.J.,
RA   Honchak B.M., Karbach L.E., Land M.L., Lapidus A., Larimer F.W.,
RA   Mikhailova N., Pitluck S., Pierson B.K., Blankenship R.E.;
RT   "Complete genome sequence of the filamentous anoxygenic phototrophic
RT   bacterium Chloroflexus aurantiacus.";
RL   BMC Genomics 12:334-334(2011).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; CP000909; ABY36645.1; -; Genomic_DNA.
DR   RefSeq; WP_012259298.1; NC_010175.1.
DR   RefSeq; YP_001637034.1; NC_010175.1.
DR   AlphaFoldDB; A9WKL7; -.
DR   SMR; A9WKL7; -.
DR   STRING; 324602.Caur_3460; -.
DR   PRIDE; A9WKL7; -.
DR   EnsemblBacteria; ABY36645; ABY36645; Caur_3460.
DR   KEGG; cau:Caur_3460; -.
DR   PATRIC; fig|324602.8.peg.3899; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_0; -.
DR   InParanoid; A9WKL7; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000002008; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..651
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000345254"
FT   BINDING         11..16
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         296..310
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   651 AA;  71884 MW;  2D8557B5C777E496 CRC64;
     MQTRYDVIVV GAGHAGCEAA HAAARLGCRT LLLTIDLDKL AHMSCNPSIG GPAKGHLVRE
     IDALGGLMGR ITDRSAIQIR LLNESKGPAV QSLRAQCDKR LYARLMKETL ERVPNLDLRQ
     AMVERIAPPN ADTQCFTVTT HTGWRYLAPA VILTTGTFLR GRAITGEAMW GAGRAGEAPA
     MALSEDLAAL GFPLVRLKTG TPPRLAAATI DFSLTELQPG SDTPLSFGHY YPELGETIPP
     PEYHGPPAPV YPHPQLDGWR PQLPCYQVHT TPEFHAIIRE NLHRAPLFSG IIEGVGPRYC
     PSIEDKIVRF ADKERHGLFL EPEGWTTSEV YVQGCNTSLP EDVQWAMLRS IPALRNVELM
     RIGYAIEYDA VATGEITADM QTRRLRGLFF AGQINGTTGY EEAAAQGLMA GINAAHYVQG
     KPPVILGRAE AYIGVLIDDL TTKEIREPYR MFTSRAEYRL LLRGDNADLR LTPLAYRLGL
     VDGERAAVVE ARRQQTEHAL QQMRERRIFP SAAVNASLEA HGIKPISQPV TVAEVLARPE
     VRYTQLRDAL PDLPALSDAV IEQVEIGCKY SGYIARQERE VARMQKMEHR RIPPDFDYTS
     LPGLRNEARQ VLMRFRPATL GQAGRLAGIN PADVAIILFA LERRQGDQVA R