ID   MNMG_CHLT2              Reviewed;         610 AA.
AC   B0B872; O84506;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=CTL0760;
OS   Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC VR-902B).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=471472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=434/Bu / ATCC VR-902B;
RX   PubMed=18032721; DOI=10.1101/gr.7020108;
RA   Thomson N.R., Holden M.T.G., Carder C., Lennard N., Lockey S.J., Marsh P.,
RA   Skipp P., O'Connor C.D., Goodhead I., Norbertzcak H., Harris B., Ormond D.,
RA   Rance R., Quail M.A., Parkhill J., Stephens R.S., Clarke I.N.;
RT   "Chlamydia trachomatis: genome sequence analysis of lymphogranuloma
RT   venereum isolates.";
RL   Genome Res. 18:161-171(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-80.
RA   Wang L., Steenburg S.D., Zheng Y., Larsen S.H.;
RT   "Gene identification of Chlamydia trachomatis by random DNA sequencing.";
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; AM884176; CAP04198.1; -; Genomic_DNA.
DR   EMBL; AF087351; AAD04123.1; -; Genomic_DNA.
DR   RefSeq; WP_009873860.1; NC_010287.1.
DR   RefSeq; YP_001654831.1; NC_010287.1.
DR   AlphaFoldDB; B0B872; -.
DR   SMR; B0B872; -.
DR   EnsemblBacteria; CAP04198; CAP04198; CTL0760.
DR   KEGG; ctb:CTL0760; -.
DR   PATRIC; fig|471472.4.peg.816; -.
DR   HOGENOM; CLU_007831_2_2_0; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000000795; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; tRNA processing.
FT   CHAIN           1..610
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000345252"
FT   BINDING         14..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         274..288
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   610 AA;  67263 MW;  AE5128768C1B6F85 CRC64;
     MWTFPVDYDV IVIGAGHAGC EAAYCAAKMG ASVLLLTSNL DTIAKLSCNP AVGGIGKGHI
     VREIDALGGI MAEITDLSGI QFRILNQTKG PAVRAPRAQV DKQLYHIHMK RLLEQVPGLH
     IMQGTAEALL DNGEKVLGVS TKEGWAYLGK TVVLSSGTFM RGLIHIGTQN FSGGRLGDAA
     SLGLSEDLKR LGFPLGRLKT GTPARLLASS IDFSVMEEQP GDHNVCFVHR NEMFVPTLPQ
     VSCHITHTTD QTKDLITKNL HRSALYGGRI EGVGPRYCPS IEDKIVKFAD KDRHHIFIEP
     EGLNTQEVYV NGLSTSMPFD VQYDIIRSVS GLENAIITRP AYAIEYDYVH GNVIFPSLES
     KLIEGLFLCG QINGTTGYEE AAAQGLIAGV NAVNKVLRRP PFVPSRQESY IGVMLDDLTT
     QVLDEPYRMF TSRAEHRLLL RQDNAGMRLS HYGHSLGLLS SERYAMFQEQ KACIEQEKER
     LSKTFRKYGD TVVPLTRVLC RPEVSYQQLL TEFPADVRDL GPIVGASLEM EIKYSGYISR
     QQTLIRSMER SENISIPEDI DYHSISALSL EAREKLSKFT PRTIGSAARI SGISVADIQV
     LMVSLKKDAH