ID   MNMG_CHLTR              Reviewed;         610 AA.
AC   P0CD73; O84506;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=CT_498;
OS   Chlamydia trachomatis (strain D/UW-3/Cx).
OC   Bacteria; Chlamydiae; Chlamydiales; Chlamydiaceae;
OC   Chlamydia/Chlamydophila group; Chlamydia.
OX   NCBI_TaxID=272561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=D/UW-3/Cx;
RX   PubMed=9784136; DOI=10.1126/science.282.5389.754;
RA   Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L.,
RA   Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.;
RT   "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia
RT   trachomatis.";
RL   Science 282:754-759(1998).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE001273; AAC68099.1; -; Genomic_DNA.
DR   PIR; H71503; H71503.
DR   RefSeq; NP_220013.1; NC_000117.1.
DR   RefSeq; WP_010725215.1; NC_000117.1.
DR   AlphaFoldDB; P0CD73; -.
DR   SMR; P0CD73; -.
DR   STRING; 813.O172_02750; -.
DR   EnsemblBacteria; AAC68099; AAC68099; CT_498.
DR   GeneID; 884271; -.
DR   KEGG; ctr:CT_498; -.
DR   PATRIC; fig|272561.5.peg.542; -.
DR   HOGENOM; CLU_007831_2_2_0; -.
DR   InParanoid; P0CD73; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000000431; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..610
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117083"
FT   BINDING         14..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         274..288
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   610 AA;  67202 MW;  6FF516182E49B49D CRC64;
     MWTFPVDYDV IVIGAGHAGC EAAYCAAKMG ASVLLLTSNL DTIAKLSCNP AVGGIGKGHI
     VREIDALGGI MAEITDLSGI QFRILNQTKG PAVRAPRAQV DKQLYHIHMK RLLEQVPGLH
     IMQGTAEALL DNGEKVLGVS TKEGWAYLGK TVVLSSGTFM RGLIHIGTQN FSGGRLGDAA
     SLGLSEDLKR LGFPLGRLKT GTPARLLASS IDFSVMEEQP GDHNVCFVHR NEMFVPTLPQ
     VSCHITHTTD QTKDLITKNL HRSALYGGRI EGVGPRYCPS IEDKIVKFAD KDRHHIFIEP
     EGLNTQEVYV NGLSTSMPFD VQYDIIRSVS GLENAIITRP AYAIEYDYVH GNVIFPSLES
     KLIEGLFLCG QINGTTGYEE AAAQGLIAGV NAVNKVLRHP PFVPSRQESY IGVMLDDLTT
     QVLDEPYRMF TSRAEHRLLL RQDNAGMRLS HYGHSLGLLS SERYAMFQEQ KACIEQEKER
     LSKTFRKYGD TVVPLTKVLC RPEVSYQQLL TEFPADVRDL GPVVGASLEM EIKYSGYISR
     QQTLIRSMER SENISIPEDI DYHSISALSL EAREKLSKFT PRTIGSAARI SGISVADIQV
     LMVSLKKDAH