ID   MNMG_HELP2              Reviewed;         621 AA.
AC   B6JKE5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=HPP12_0214;
OS   Helicobacter pylori (strain P12).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=570508;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=P12;
RA   Fischer W., Windhager L., Karnholz A., Zeiller M., Zimmer R., Haas R.;
RT   "The complete genome sequence of Helicobacter pylori strain P12.";
RL   Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; CP001217; ACJ07373.1; -; Genomic_DNA.
DR   RefSeq; WP_000238043.1; NC_011498.1.
DR   AlphaFoldDB; B6JKE5; -.
DR   SMR; B6JKE5; -.
DR   EnsemblBacteria; ACJ07373; ACJ07373; HPP12_0214.
DR   KEGG; hpp:HPP12_0214; -.
DR   HOGENOM; CLU_007831_2_2_7; -.
DR   OMA; FRPGYAI; -.
DR   OrthoDB; 146811at2; -.
DR   Proteomes; UP000008198; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; tRNA processing.
FT   CHAIN           1..621
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_1000095652"
FT   BINDING         11..16
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         270..284
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   621 AA;  69598 MW;  6EE370C25BB5F009 CRC64;
     MVKESDILVI GGGHAGIEAS LIAAKMGARV HLITMLIDTI GLASCNPAIG GLGKGHLTKE
     VDVLGGAMGI ITDNSGLQYR VLNASKGPAV RGTRAQIDMD TYRILARNLV LNTPNLSVSQ
     EMTESLIVEN GEIVGVTTNI NNTYKAKKVI ITTGTFLKGV VHIGEHQNQN GRFGENASNS
     LALNLRELGF KVERLKTGTC PRVAGNSIDF EGLEEHFGDT NPPYFSYKTK DFNPTQLSCF
     ITYTNPITHQ IIRDNFHRAP LFSGQIEGIG PRYCPSIEDK INRFSEKERH QLFLEPQTIH
     KSEYYINGLS TSLPLDVQEK VIHSIKGLEN ALITRYGYAI EYDFIQPTEL THTLETKKIK
     GLYLAGQING TTGYEEAAAQ GLMAGINAVL ALKNQAPFIL KRNEAYIGVL IDDLVTKGTN
     EPYRMFTSRA EYRLLLREDN TLFRLGEHAY RLGLMEEDFY KELKKDQQAI QENLKRLREC
     VLTPSKEVLK RLNELDENPI NDKVDGVSLL ARDSFNLEKM RSFFSFLAPL NERVLEQIKI
     ECKYNIYIEK QHENIAKMDS MLKVSIPKDF VFKGIPGLSL EAVEKLEKFR PKSLFEASEI
     SGITPANLDV LHLYIHLRKN S