ID   MNMG_LEPIN              Reviewed;         635 AA.
AC   Q8EY59;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=LA_4359;
OS   Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain
OS   56601).
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=189518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=56601;
RX   PubMed=12712204; DOI=10.1038/nature01597;
RA   Ren S.-X., Fu G., Jiang X.-G., Zeng R., Miao Y.-G., Xu H., Zhang Y.-X.,
RA   Xiong H., Lu G., Lu L.-F., Jiang H.-Q., Jia J., Tu Y.-F., Jiang J.-X.,
RA   Gu W.-Y., Zhang Y.-Q., Cai Z., Sheng H.-H., Yin H.-F., Zhang Y., Zhu G.-F.,
RA   Wan M., Huang H.-L., Qian Z., Wang S.-Y., Ma W., Yao Z.-J., Shen Y.,
RA   Qiang B.-Q., Xia Q.-C., Guo X.-K., Danchin A., Saint Girons I.,
RA   Somerville R.L., Wen Y.-M., Shi M.-H., Chen Z., Xu J.-G., Zhao G.-P.;
RT   "Unique physiological and pathogenic features of Leptospira interrogans
RT   revealed by whole-genome sequencing.";
RL   Nature 422:888-893(2003).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; AE010300; AAN51557.1; -; Genomic_DNA.
DR   RefSeq; NP_714539.1; NC_004342.2.
DR   RefSeq; WP_000572982.1; NC_004342.2.
DR   AlphaFoldDB; Q8EY59; -.
DR   SMR; Q8EY59; -.
DR   STRING; 189518.LA_4359; -.
DR   EnsemblBacteria; AAN51557; AAN51557; LA_4359.
DR   KEGG; lil:LA_4359; -.
DR   PATRIC; fig|189518.3.peg.4325; -.
DR   HOGENOM; CLU_007831_2_2_12; -.
DR   InParanoid; Q8EY59; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000001408; Chromosome I.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR   GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..635
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117123"
FT   BINDING         20..25
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         279..293
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   635 AA;  71947 MW;  D4139B3A93230270 CRC64;
     MIESKNQSFF PNRFDCVVVG AGHAGSEAAY ISSKGGARTL LITMNLDTIG QMSCNPAIGG
     IAKGHMVREV DALGGIMGKM IDNTGIQFKM LNTSKGPSVW APRAQAEKKE YQLKVKHTLE
     AEKNLSIRQD TVEELIIEND QVIGVRTGRG FEIFTDHVIL TTGTFLSSLV HIGTYQNENG
     RMCEPTVKGL SKSLAKYNLK LGRLKTGTPP RIHKNSVDLS VLTIQEGDSN PSPFSFSTDK
     ITRKQIPCFI TYTNAETHKL IHENLNLSPM YSGQIQSTGP RYCPSIEDKV VRFADRERHQ
     VFLEPEGYET SEIYLNGVST SLPEEVQWKL VRSLKGLENA EIVRPGYAIE YDYVDPTELK
     PTLETKKIKG LYHAGQINGT TGYEEAAAQG LVAAYSVLHS LRNLTPLLFK RSESYIGVLI
     DDLVHKGVED PYRMFTSRAE HRLLLRQDNA DQRLMKYGYD LGLVDQKSYD CMKEKYERVN
     SVREKIYQIP LKPSDKFQNL LDQKGITNYK FGMKLDSFLK RPEIKIKDIE FMIPEVSSWS
     DLEKSILEME IKYEGYIKRE LETIQWKNKY LDLAIPEDIN YEMIAGLKKE AIQKLKSHKP
     MTLEKASQIS GVDPSDVDLI LYHIKGKKKQ EVEIF