ID   MNMG_MESFL              Reviewed;         626 AA.
AC   Q6F0E6;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=Mfl671;
OS   Mesoplasma florum (strain ATCC 33453 / NBRC 100688 / NCTC 11704 / L1)
OS   (Acholeplasma florum).
OC   Bacteria; Tenericutes; Mollicutes; Entomoplasmatales; Entomoplasmataceae;
OC   Mesoplasma.
OX   NCBI_TaxID=265311;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33453 / NBRC 100688 / NCTC 11704 / L1;
RA   Birren B.W., Stange-Thomann N., Hafez N., DeCaprio D., Fisher S.,
RA   Butler J., Elkins T., Kodira C.D., Major J., Wang S., Nicol R., Nusbaum C.;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017263; AAT76027.1; -; Genomic_DNA.
DR   RefSeq; WP_011183567.1; NC_006055.1.
DR   RefSeq; YP_053911.1; NC_006055.1.
DR   AlphaFoldDB; Q6F0E6; -.
DR   SMR; Q6F0E6; -.
DR   STRING; 265311.Mfl671; -.
DR   PRIDE; Q6F0E6; -.
DR   EnsemblBacteria; AAT76027; AAT76027; Mfl671.
DR   GeneID; 2898043; -.
DR   KEGG; mfl:Mfl671; -.
DR   PATRIC; fig|265311.5.peg.673; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_14; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000006647; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..626
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117129"
FT   BINDING         11..16
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         272..286
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   626 AA;  69915 MW;  3291C431D7BE8546 CRC64;
     MNKHFDVIVI GGGHAGVEAA LASARLNKKT ALINLYKDRI AAMPCNPSVG GPAKGIVVRE
     IDALGGEMAK AADKTALQTK LLNSSRGPGV WALRVQSDKE EYSKYMQKIV EEQINLQLIE
     SIATDLIIEN NTVTGVLLKD GEILKSKCVV LTTGTYLKSE ILTGHEKFSS GPNEEPTSNG
     ISESLKKSGV ELFRFKTGTP PRIYKNSVDL SKAIKEPGTD AKLAFSFSTN EYTPIEKQEL
     CYLIHSSSET KKIIEDNLTK SAMYSGKVES IGPRYCPSFE DKIVRFSSKE THQIFIEPES
     ISGDAWYIQG FSTSMPTDVQ LLMIRSLPGF EKAEVKTWAY AIEYDCVNPM QLNPSLEIKN
     INNLFTAGQI NGTSGYEEAA GQGLIAGINA SRKIDGLDPL ILRRDEAYIG VMIDDLINKG
     VWEPYRLLTS RAEHRLLLRN DNAEHRLKEK GYEIGLIKQA EYNEYLKYTE EINNAIKELE
     EIRFTPKSEL AIKLKELGQA DLSHGYSGIE ILKIPTVDIE VMIPYISSLQ NLKNNQLQSI
     VIETRFAGYV KTERKTVERL VKLEKKKIPS DIDYDKVENL ATEARQKLKK VKPLNIGQAS
     RITGVNPADI QMLLFYLKNN YATEQK