MNMG_MYCGE
ID MNMG_MYCGE Reviewed; 612 AA.
AC P47619;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=MG379;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 301-394.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; L43967; AAC71606.1; -; Genomic_DNA.
DR EMBL; U01812; AAD12347.1; -; Genomic_DNA.
DR PIR; I64241; I64241.
DR RefSeq; WP_010869458.1; NC_000908.2.
DR AlphaFoldDB; P47619; -.
DR SMR; P47619; -.
DR STRING; 243273.MG_379; -.
DR PRIDE; P47619; -.
DR EnsemblBacteria; AAC71606; AAC71606; MG_379.
DR KEGG; mge:MG_379; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_14; -.
DR OMA; FRPGYAI; -.
DR OrthoDB; 146811at2; -.
DR BioCyc; MGEN243273:G1GJ2-473-MON; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..612
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_0000117132"
FT BINDING 9..14
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 270..284
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 612 AA; 68995 MW; 67A3F64C985B5912 CRC64;
MSFIITVIGA GHAGLEAAFI VSKFNIKVNL LVLDINHLGS CPCNPSIGGP AKGIVTREID
VLGGMQAIAA DNNALQYKLL NSSKGPAVQA IRAQIDKIGY KNWFQSQVKL NKNINLIQSE
AINLIVRNEK IKGVILKDGS ELLSDAVIIT TGTYLRSKTY CGNTVKNQGP DQSKNSEKLS
TNLINRGFKT IRLKTGTPPR ILKTSLDYNQ MELEINNNQN LAFSTTNKNF LPLEKQIPCY
LVHTNQKIHD LILKNLKKSA MFNGSISAQG PLYCPSIEDK VFKFSQKPRH QIFVEPESLS
LDTIYLAGLS TSFTPEIQKE IIQLLPGFQN AEIKKFGYAI EYDAFLSNQL KPTLETKLIE
NLYFAGQING TSGYEEAAGQ GLMAGINAAL KLLKKPPFIL QRNEAYIGVM INDLVTKTIS
DPYRLLTSRA EYRLWLRNDN VQERLIKKSF ELGLTDKKTY ELFLKKEKKK QELISFLKNT
QVGKVKALKF TNKNTAQSLY DFNKRSEINL DKLIKDLPEK YQLDSETLKQ IEIEIKYEGY
IKKNEKYFKG LDKLSKIKIP HTFDYHKVKN LASEAIFKLS NFKPSNLAIA SQIAGVNFND
IIAIKHFLKT YE
