MNMG_OPITP
ID MNMG_OPITP Reviewed; 634 AA.
AC B1ZWP4;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-JUL-2008, sequence version 2.
DT 03-AUG-2022, entry version 69.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN OrderedLocusNames=Oter_0883;
OS Opitutus terrae (strain DSM 11246 / JCM 15787 / PB90-1).
OC Bacteria; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus.
OX NCBI_TaxID=452637;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 11246 / JCM 15787 / PB90-1;
RX PubMed=21398538; DOI=10.1128/jb.00228-11;
RA van Passel M.W., Kant R., Palva A., Copeland A., Lucas S., Lapidus A.,
RA Glavina del Rio T., Pitluck S., Goltsman E., Clum A., Sun H., Schmutz J.,
RA Larimer F.W., Land M.L., Hauser L., Kyrpides N., Mikhailova N.,
RA Richardson P.P., Janssen P.H., de Vos W.M., Smidt H.;
RT "Genome sequence of the verrucomicrobium Opitutus terrae PB90-1, an
RT abundant inhabitant of rice paddy soil ecosystems.";
RL J. Bacteriol. 193:2367-2368(2011).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACB74171.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP001032; ACB74171.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_044891563.1; NC_010571.1.
DR AlphaFoldDB; B1ZWP4; -.
DR SMR; B1ZWP4; -.
DR STRING; 452637.Oter_0883; -.
DR EnsemblBacteria; ACB74171; ACB74171; Oter_0883.
DR KEGG; ote:Oter_0883; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_0; -.
DR OrthoDB; 146811at2; -.
DR Proteomes; UP000007013; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..634
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_0000345310"
FT BINDING 14..19
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 292..306
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 634 AA; 69723 MW; 0E75740F77C11E01 CRC64;
MRFNSEPFDV IVCGAGHAGI EASLAAARVG AFTLLLTGNL DTVGQMSCNP AIGGQAKGQI
VREIDALGGE MAINTDVTGI QFRLLNHTKG PAVQSPRAQC DKKAYQFRLK HTLELQPHLQ
LFQATVTGLI FDGAKVIGCR TNLDIEFYGR TVVVTTGTFL RGLMHIGSNK NEGGRLGDFS
ARTLSGSLQE AGIELRRFKT GTPPRLLGRS LDFSKMEEQK GDANPTFFAF HDTREDGGVF
HVEHGKPQGE AIPPGCEQVS CWMTYTTART AELVRENLSR SAMYGGEIEG VGPRYCPSIE
DKFVRFADKP RHLLFLEPEG RGTDEFYVNG LSTSLPFDVQ LELVHSIPGL GEAVLLRPAY
AVEYDYAPPT QLKMSLESQR VEGLFLAGQI NGTSGYEEAA GQGLIAGVNA ARKGRGEQPL
VLGRHEAYIG VLIDDLVTKG TEEPYRMFTS RAEHRLLLNH GSAELRLLHH ASGCKLLNVN
RLDNIARKKE AIESWVRRLE SERTSEGTLA EIIRRQNARA ELPSELMRQS KPVRDEVLYR
VLYSGYWERE QRHIDKLAQI EAVRIPAAID YLRIPGLRRE SALKLAAQRP DTLGQASRIS
GVNPADVSIL MVLIKTGKTP SLPDTATTDG RDVP