MNMG_PROMA
ID MNMG_PROMA Reviewed; 667 AA.
AC Q7V9J7;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN OrderedLocusNames=Pro_1836;
OS Prochlorococcus marinus (strain SARG / CCMP1375 / SS120).
OC Bacteria; Cyanobacteria; Synechococcales; Prochlorococcaceae;
OC Prochlorococcus.
OX NCBI_TaxID=167539;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SARG / CCMP1375 / SS120;
RX PubMed=12917486; DOI=10.1073/pnas.1733211100;
RA Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M.,
RA Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S.,
RA Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J.,
RA Tandeau de Marsac N., Weissenbach J., Wincker P., Wolf Y.I., Hess W.R.;
RT "Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a
RT nearly minimal oxyphototrophic genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; AE017126; AAQ00880.1; -; Genomic_DNA.
DR RefSeq; NP_876227.1; NC_005042.1.
DR RefSeq; WP_011125985.1; NC_005042.1.
DR AlphaFoldDB; Q7V9J7; -.
DR SMR; Q7V9J7; -.
DR STRING; 167539.Pro_1836; -.
DR PRIDE; Q7V9J7; -.
DR EnsemblBacteria; AAQ00880; AAQ00880; Pro_1836.
DR GeneID; 54201166; -.
DR KEGG; pma:Pro_1836; -.
DR PATRIC; fig|167539.5.peg.1938; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_3; -.
DR OMA; FRPGYAI; -.
DR OrthoDB; 146811at2; -.
DR Proteomes; UP000001420; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..667
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_0000117150"
FT BINDING 17..22
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 293..307
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 667 AA; 74462 MW; 16321F72BFAA0BC6 CRC64;
MSNYSNAKEK FDVIVVGGGH AGCEAAIATA KLGLSTALFT LNLDRIAWQP CNPAVGGPAK
SQLVHEVDAL GGVIGRLADS TALQKRILNA SRGPAVWALR AQTDKRQYSI EMLQMLQETP
NLSLREAMVT DLEIKSSPNR KKIKEQSEEK TIGQVQGIKT YFGSIYLAKA VILTTGTFLK
GKIWIGNQSM DAGRAGEQAA TGLTEALQKL GFETSRLKTG TPPRVDRRTI DLGSLEEQKS
DAADRFFSFD PERWKSGQQM SCYMTRTTQK THQLIKNNLH LTPIYGGFIN SKGPRYCPSI
EDKIVRFSDK ESHQIFLEPE GRNTPEIYIQ GLSTGLPEGM QLQLLRTLPG LKNCVMLRPA
YAVEYDYLPA TQLLASLETK KIEGLFSAGQ LNGTTGYEEA AAQGLVAGLN AARYVNNQDA
IIFPREESYI GTMIDDLIKK DLREPYRVLT SRSEYRLILR GDNADRRLTP LGYKVGLVND
YQWNIFQIKQ SNMEKEKKRL EQQRIKDSDP IALKIEKESG ASIKGSITLA NLLRRPGMHM
NNLIRHKLAN NSLPLDVREG VEIDIKYSGY LKRQNLQIEQ IKKQNKRALP SEVNYTNIKT
LSQEAREKLN ATQPKTFGEA SQLPGVSKAD LSALLIWLKI QNRKSHAAKI RNNEAKIKLH
SNLGVDF
