MNMG_PSEAE
ID MNMG_PSEAE Reviewed; 630 AA.
AC Q9HT09;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=PA5565;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE004091; AAG08950.1; -; Genomic_DNA.
DR PIR; D82950; D82950.
DR RefSeq; NP_254252.1; NC_002516.2.
DR RefSeq; WP_003114649.1; NZ_QZGE01000012.1.
DR AlphaFoldDB; Q9HT09; -.
DR SMR; Q9HT09; -.
DR STRING; 287.DR97_2944; -.
DR PaxDb; Q9HT09; -.
DR PRIDE; Q9HT09; -.
DR EnsemblBacteria; AAG08950; AAG08950; PA5565.
DR GeneID; 877676; -.
DR KEGG; pae:PA5565; -.
DR PATRIC; fig|208964.12.peg.5831; -.
DR PseudoCAP; PA5565; -.
DR HOGENOM; CLU_007831_2_2_6; -.
DR InParanoid; Q9HT09; -.
DR OMA; FRPGYAI; -.
DR PhylomeDB; Q9HT09; -.
DR BioCyc; PAER208964:G1FZ6-5692-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; ISS:PseudoCAP.
DR GO; GO:0030488; P:tRNA methylation; ISS:PseudoCAP.
DR GO; GO:0002098; P:tRNA wobble uridine modification; ISS:PseudoCAP.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..630
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_0000117153"
FT BINDING 13..18
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 273..287
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 630 AA; 69638 MW; 68FF9F114676A9B6 CRC64;
MDFPTRFDVI VIGGGHAGTE AALAAARMGV KTLLLTHNVE TLGQMSCNPA IGGIGKSHLV
KEIDALGGAM AEATDKGGIQ FRILNSRKGP AVRATRAQAD RVLYKAAIRH TLENQPNLWI
FQQACDDLIV EQDQVRGVVT QMGLRFHADN VVLTTGTFLG GLIHIGLENY SGGRAGDPPS
IALARRLREL PLRVGRLKTG TPPRIDGRSV DFSVMTEQPG DTPIPVMSFL GSKEQHPEQV
SCWITHTNAR THEIIAANLD RSPMYSGVIE GIGPRYCPSI EDKIHRFADK ESHQVFLEPE
GLTTHELYPN GISTSLPFDV QLQIVRSIRG MENAHIVRPG YAIEYDFFDP RDLRYSLETK
VIGGLFFAGQ INGTTGYEEA GAQGLLAGAN AALRSQGKDS WCPRRDEAYI GVLVDDLITL
GTQEPYRMFT SRAEYRLILR EDNADLRLTE KGRELGLVDD RRWAAFEAKR EGIEREEQRL
KSTWVRPNTP QGDAIAERFG TPLTHEYNLL NLLSRPEIDY AGLVEITGNA VDNPQVAEQV
EIRTKYAGYI DRQQEEISRL RASEDTRLPV DIDYLGISGL SKEIQNKLNQ ARPETLGQAS
RIPGVTPAAI SLLLIHLKKR ASGRQLEQSA
