ID   MNMG_PSEU2              Reviewed;         631 AA.
AC   Q9F5X1; Q4ZL13;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=Psyr_5132;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kinscherf T.G., Willis D.K.;
RT   "Global regulatory effects associated with gidA mutations in Pseudomonas
RT   syringae.";
RL   Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAY40159.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF302083; AAG22815.1; -; Genomic_DNA.
DR   EMBL; CP000075; AAY40159.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_238197.1; NC_007005.1.
DR   AlphaFoldDB; Q9F5X1; -.
DR   SMR; Q9F5X1; -.
DR   STRING; 205918.Psyr_5132; -.
DR   EnsemblBacteria; AAY40159; AAY40159; Psyr_5132.
DR   KEGG; psb:Psyr_5132; -.
DR   PATRIC; fig|205918.7.peg.5293; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_6; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; tRNA processing.
FT   CHAIN           1..631
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000117157"
FT   BINDING         14..19
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         274..288
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   631 AA;  69402 MW;  2B3B1BC73BB883E1 CRC64;
     MVDFPSRFEV IVIGGGHAGT EAALASARMG VKTLLLTHNV ETLGQMSCNP AIGGIGKSHL
     VKEIDALGGA MAMATDKGGI QFRVLNSRKG PAVRATRAQA DRVLYKAAIR ETLENQPNLW
     IFQQACDDLI VDQDQVRGVV TQMGMRIFAD SVVLTTGTFL GGLIHIGMQN YSGGRAGDPP
     SIALAQRLRE LPLRVGRLKT GTPPRIDGRS VDFSVMTEQP GDTPIPVMSF LGNKEQHPPQ
     VSCWITHTNA RTHEIIASNL DRSPMYSGVI EGIGPRYCPS IEDKIHRFAD KESHQVFIEP
     EGLTTHELYP NGISTSLPFD VQLQIVRSIR GMENAHIVRP GYAIEYDYFD PRDLKYSLET
     KVIGGLFFAG QINGTTGYEE AGAQGLLAGT NAALRAQGRD SWCPRRDEAY IGVLVDDLIT
     LGTQEPYRMF TSRAEYRLIL REDNADLRLT EKGRELGLVD DARWAAFCIK RESIELEEQR
     LKSTWVRPGT EQGDAIAAHF GTPLTHEYNL LNLLTRPEID YASLISITGQ GCTDPQVAEQ
     VEIKTKYAGY IDRQQDEIAR LRASEDTRLP EDIDYAAISG LSKEIQSKLG ITRPETLGQA
     SRIPGVTPAA ISLLMIHLKK RGAGRQLEQS A