ID   MNMG_ROSS1              Reviewed;         679 AA.
AC   A5UY26;
DT   22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=RoseRS_3168;
OS   Roseiflexus sp. (strain RS-1).
OC   Bacteria; Chloroflexi; Chloroflexia; Chloroflexales; Roseiflexineae;
OC   Roseiflexaceae; Roseiflexus.
OX   NCBI_TaxID=357808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RS-1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Bryant D.A., Richardson P.;
RT   "Complete sequence of Roseiflexus sp. RS-1.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; CP000686; ABQ91529.1; -; Genomic_DNA.
DR   RefSeq; WP_011957873.1; NC_009523.1.
DR   AlphaFoldDB; A5UY26; -.
DR   SMR; A5UY26; -.
DR   STRING; 357808.RoseRS_3168; -.
DR   PRIDE; A5UY26; -.
DR   EnsemblBacteria; ABQ91529; ABQ91529; RoseRS_3168.
DR   KEGG; rrs:RoseRS_3168; -.
DR   eggNOG; COG0445; Bacteria.
DR   HOGENOM; CLU_007831_2_2_0; -.
DR   OMA; FRPGYAI; -.
DR   OrthoDB; 146811at2; -.
DR   Proteomes; UP000006554; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT   CHAIN           1..679
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_0000345327"
FT   BINDING         15..20
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         314..328
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   679 AA;  74906 MW;  908A19076A023561 CRC64;
     MHSTHPYIYD VIVVGAGHAG CEAAHAAARM GCRTLLLTID LDKLAHMSCN PSIGGPAKGH
     LVREIDALGG LMGRVTDRTF IQIRLLNESK GPAVQAPRAQ ADKRLYAKVM KETLETIPNL
     DLRQAMIERI ILPRPINRNG SDTDDAEALC PGHYAVVTHT RRIYQCRALV LTTGTFLRGR
     AITGDAIWGA GRAGEAPATA LGEDLAALGF PLVRLKTGTP PRIDARTIDF SLTSVQHGSA
     TPLFFGFYYR ALNEAPPEPA FGGPPASAYP EPLLDAWRPQ LPCYLVHTTP EFHEIVRRNL
     DRAPLFSGII EGVGPRYCPS IEDKIVRFAD KERHGLFLEP EGWSTHEVYV QGCNTSLPED
     VQWAMLRSIP ALRRVELMRA GYAIEYDALA TGEIAADMSS RRAPGLFFAG QINGTTGYEE
     AAAQGLMAGI NAARFVQGKP SVLLRRDEAY IGVLIDDLVT KEIREPYRMF TSRAEHRLLL
     RADNADLRLT PLAGELGLVD RERVAVVERK REEVERLLAV LRGKRLYPSA AINARLSAAG
     IAPLTGEMSA EEVLRRPEVR YRQLQPALDL PACEADVAEQ VDIEAKYSGY LLKQQREVER
     LRRMESRRIP PDFDFAALRG LRNEARQTLQ RFRPATIGQA ARLAGINPAD IALLLVALER
     QTRQATPVET PHPSLPHTG