MNMG_SYNSC
ID MNMG_SYNSC Reviewed; 643 AA.
AC Q3AGK9;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN OrderedLocusNames=Syncc9605_2545;
OS Synechococcus sp. (strain CC9605).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=110662;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC9605;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Schmutz J., Martinez M., Larimer F.,
RA Land M., Kyrpides N., Ivanova N., Richardson P.;
RT "Complete sequence of Synechococcus sp. CC9605.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000110; ABB36273.1; -; Genomic_DNA.
DR RefSeq; WP_011365468.1; NC_007516.1.
DR AlphaFoldDB; Q3AGK9; -.
DR SMR; Q3AGK9; -.
DR STRING; 110662.Syncc9605_2545; -.
DR PRIDE; Q3AGK9; -.
DR EnsemblBacteria; ABB36273; ABB36273; Syncc9605_2545.
DR KEGG; syd:Syncc9605_2545; -.
DR eggNOG; COG0445; Bacteria.
DR HOGENOM; CLU_007831_2_2_3; -.
DR OMA; FRPGYAI; -.
DR OrthoDB; 146811at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; tRNA processing.
FT CHAIN 1..643
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_1000016703"
FT BINDING 17..22
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 281..295
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 643 AA; 70256 MW; 0CA51D969ADE687E CRC64;
MSFSAAPTES FDVIVVGGGH AGCEAAITAA RLGLNTALFS LNLDRIAWQP CNPAVGGPAK
SQLVHEVDAL GGVIGRLADA TAIQKRILNA SRGPAVWALR AQTDKRLYSR QMLQLLQHTP
NLALREAMVT GLETTVAGDQ QRISGIRTYF GSVYGADAVI LTAGTFLGGR IWVGHQSMAA
GRAGEQAAEG LTETLQELGF QTDRLKTGTP ARVDRRSIAL DQLEEQPSDA ADRFFSFDPA
AWVSGEQMSC HITRTTAETH QLIRDNLHLT AIYGGVIDSK GPRYCPSIED KIVRFADKDS
HQIFLEPEGR DTPEIYVQGF STGLPEPIQL QLLRSLPGLE QAVMLRPAYS VDYDYLPATQ
LKPSLETKRV RGLFSAGQLN GTTGYEEAAA QGLVAGVNAG RLIGGQEPVH FPREGSYIGT
MIDDLVSKDL REPYRVLTSR SEYRLILRGD NADRRLTPLG HELGLIDDRR WQLFEDKLQA
MEGEKQRLET VRLKVSDPVA PAVEQETGAA IKGSITLADL LRRPGMHAAD LVRHGLADAD
LPLPVREGAE IDIKYSGYLQ RQQQQIDQVK RQSQRKLPAD LNYAGIGTLS NEAREKLTAI
QPSTLGQASR IPGVSKADIT ALLMWLELQQ RERQPLAPTA EAR